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Titolo:
Peripheral nerve protein, P0, as a potential receptor for Theiler's murineencephalomyelitis virus
Autore:
Libbey, JE; McCright, IJ; Tsunoda, I; Wada, Y; Fujinami, RS;
Indirizzi:
Univ Utah, Sch Med, Dept Neurol, Salt Lake City, UT 84132 USA Univ Utah Salt Lake City UT USA 84132 eurol, Salt Lake City, UT 84132 USA Jackson Lab, Gene Express Database, Mouse Genome Informat, Bar Harbor, ME 04609 USA Jackson Lab Bar Harbor ME USA 04609 me Informat, Bar Harbor, ME 04609 USA Nissan Tamagawa Hosp, Dept Neurol, Setagaya Ku, Tokyo, Japan Nissan Tamagawa Hosp Tokyo Japan Dept Neurol, Setagaya Ku, Tokyo, Japan
Titolo Testata:
JOURNAL OF NEUROVIROLOGY
fascicolo: 2, volume: 7, anno: 2001,
pagine: 97 - 104
SICI:
1355-0284(200104)7:2<97:PNPPAA>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
MYELIN PO-PROTEIN; IMMUNOGLOBULIN SUPERFAMILY; ADHESION MOLECULES; CELLULAR RECEPTOR; GDVII STRAIN; INFECTION; SYSTEM; POLIOVIRUS; GENE; EXPRESSION;
Keywords:
Cardiovirus infections; myelin P0 protein; peripheral nerves; Picornaviridae infections; virus receptors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Fujinami, RS Univ Utah, Sch Med, Dept Neurol, 30N 1900E,3R330, Salt Lake City, UT 84132USA Univ Utah 30N 1900E,3R330 Salt Lake City UT USA 84132 4132USA
Citazione:
J.E. Libbey et al., "Peripheral nerve protein, P0, as a potential receptor for Theiler's murineencephalomyelitis virus", J NEUROVIRO, 7(2), 2001, pp. 97-104

Abstract

Theiler's murine encephalomyelitis virus (TMEV) belongs the family Picornaviridae. TMEV not only replicates in the gastrointestinal tract but also spreads to the central nervous system (CNS) either by a hematogenous or a neural pathway during natural infection. The DA strain of TMEV infects neuronsduring the acute phase, and glial cells and macrophages during the chronicphase, leading to a demyelinating disease similar to multiple sclerosis, Different virus-host receptor interactions in the peripheral and the neuronal cells could explain the pathways of viral spread from the peripheral to the CNS and neurons to glial cells. However, the receptor for TMEV remains unknown, PO protein, a 28-31 kD glycoprotein, belongs to the immunoglobulin superfamily and constitutes 50% of the total myelin protein in the peripheral nerve. Other picornaviruses use members of the immunoglobulin superfamily as receptors. Thus we hypothesized PO protein could act as a receptor forTMEV. In a virus overlay assay, radiolabeled TMEV bound to a 28-30 kD protein from the peripheral nerve of wild-type C57BL/6, but no binding was found in the peripheral nerve from PO-knockout mice. TMEV replicated fourfold higher in PO-transfected BW5147.G.1.4 cells than in mock-transfected cells. The increase in virus replication in the PO-transfected cell line was blocked by preincubation of the cells with anti-PO antibody. A virus binding study showed that TMEV bound to PO-transfected cells but not to mock-transfected cells. The use of the PO protein in Schwann cells as a receptor may be one mechanism by which TMEV spreads from the gastrointestinal tract to the CNS.

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Documento generato il 05/04/20 alle ore 06:43:17