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Titolo:
NMR and isotope ratio mass spectrometry studies of in vivo uptake and metabolism of polyunsaturates by the developing rat brain
Autore:
Cunnane, SC; Nadeau, CR; Likhodii, SS;
Indirizzi:
Univ Toronto, Dept Nutr Sci, Toronto, ON M5S 3E2, Canada Univ Toronto Toronto ON Canada M5S 3E2 r Sci, Toronto, ON M5S 3E2, Canada
Titolo Testata:
JOURNAL OF MOLECULAR NEUROSCIENCE
fascicolo: 2-3, volume: 16, anno: 2001,
pagine: 173 - 180
SICI:
0895-8696(200104/06)16:2-3<173:NAIRMS>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
FATTY-ACID COMPOSITION; LINOLENIC ACIDS; SUCKLING RAT; LIVER; ASTROCYTES; OXIDATION; NEURONS; DIET;
Keywords:
polyunsaturates; carbon-13; stable isotopes; nuclear magnetic resonance spectroscopy; alpha-linolenate; docosahexaenoate; arachidonate; cholesterol; isotope ratio mass spectrometry; brain;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Cunnane, SC Univ Toronto, Dept Nutr Sci, 100 Coll St, Toronto, ON M5S 3E2,Canada Univ Toronto 100 Coll St Toronto ON Canada M5S 3E2 3E2, Canada
Citazione:
S.C. Cunnane et al., "NMR and isotope ratio mass spectrometry studies of in vivo uptake and metabolism of polyunsaturates by the developing rat brain", J MOL NEURO, 16(2-3), 2001, pp. 173-180

Abstract

This article describes the application of in vivo C-13-nuclear magnetic resonance (NMR) spectroscopy and gas chromatography (CC)-combustion-isotope ratio mass spectrometry to the study of brain uptake and metabolism of polyunsaturated fatty acids in the suckling rat model. NMR spectroscopy is uniquely suited to the noninvasive detection of nonradioactive metabolites in living animals. We applied this approach to the noninvasive detection of C-13-arachidonate in brain and liver of living suckling rats but found that technical limitations in our model, mainly poor signal-to-noise, largely prevent useful results at this time. However, in a tracer study using simultaneous doses of C-13-gamma -linolenate and C-13-arachidonate, C-13-NMR of tissue lipid extracts quantitatively demonstrated a 10-fold greater (liver) or 17-fold greater (brain) accumulation of pre-formed vs newly synthesized arachidonate. CC-combustion-isotope ratio mass spectrometry was used to trace the utilization of [U-C-13]-alpha -linolenate into three products in the brain: docosahexaenoate, cholesterol, and palmitate. The rationale was that although alpha -linolenate is used in de novo lipogenesis, the quantitative importance of this pathway is unknown. Our results in the suckling rat show that 2-13% of carbon from [U-C-13]-alpha -linolenate appearing in brain lipids is in docosahexaenoate while the rest is in brain lipids synthesized denovo. Overall, these results indicate that the suckling rat brain prefers pre-formed to newly synthesized arachidonate and that alpha -linolenate is readily utilized in brain lipid synthesis. These methods are suited to comparative studies of the metabolism of polyunsaturates and they support previous observations that the metabolism of some polyunsaturates such as alpha -linolenate extends well beyond the traditional desaturation-chain elongation pathway.

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Documento generato il 21/09/20 alle ore 12:20:58