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Titolo:
'Second-look' cytotoxicity: an evaluation of culture plus cytotoxin assay of Clostridium difficile isolates in the laboratory diagnosis of CDAD
Autore:
Bouza, E; Pelaez, T; Alonso, R; Catalan, P; Munoz, P; Creixems, MR;
Indirizzi:
Hosp Gen Univ Gregorio Maranon, Serv Microbiol Clin & Enfermedades Infecciosas, Dept Clin Microbiol & Infect Dis, Madrid 28007, Spain Hosp Gen Univ Gregorio Maranon Madrid Spain 28007 s, Madrid 28007, Spain
Titolo Testata:
JOURNAL OF HOSPITAL INFECTION
fascicolo: 3, volume: 48, anno: 2001,
pagine: 233 - 237
SICI:
0195-6701(200107)48:3<233:'CAEOC>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTIBIOTIC-ASSOCIATED COLITIS; HOSPITALIZED-PATIENTS; DIARRHEA; EPIDEMIOLOGY; MULTICENTER; ENVIRONMENT; INFECTION;
Keywords:
Clostridium difficile; diarrhoea; CDAD; cytotoxicity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Alonso, R Hosp Gen Univ Gregorio Maranon, Serv Microbiol Clin & Enfermedades Infecciosas, Dept Clin Microbiol & Infect Dis, C Dr Esquerdo 46, Madrid 28007, Spain Hosp Gen Univ Gregorio Maranon C Dr Esquerdo 46 Madrid Spain 28007
Citazione:
E. Bouza et al., "'Second-look' cytotoxicity: an evaluation of culture plus cytotoxin assay of Clostridium difficile isolates in the laboratory diagnosis of CDAD", J HOSP INF, 48(3), 2001, pp. 233-237

Abstract

Clostridium difficile is one of the most frequent causes of hospital-acquired diarrhoea. Our objective was to prove that some stool samples with a direct negative cytotoxicity assay mag; indeed harbour toxigenic C. difficileand that this can be demonstrated by performing a 'second-look' cytotoxicity assay using the isolated C. difficile strains. Over an eight-year period (1992-1999), the 8241 stool samples submitted for direct cell culture from patients with suspected C. difficile-associated diarrhoea (CDAD) were simultaneously plated on cycloserine cefoxitin fructose agar. C. difficile strains isolated from samples with a negative direct cell culture assay were re-tested for toxin production ('second-look' cell culture assay). Using both methods 6423 samples (78%) were negative. Of theremaining 1818 samples, 127 (7%) yielded C. difficile isolates which were confirmed as non-producers of toxin by both methods, 1437 (85%) were positive in direct cell culture assay; and 254 were positive only after the 'second-look' cell culture assay: Thus, our approach allowed us to detect an extra 15% of toxin-producing strains that could have gone undetected otherwise. The combination of direct-cell culture assay culture for toxigenic C. difficile and 'second-look' cell culture assay enhances the potential for diagnosis of CDAD and enables us to be more efficient with our patient care resources. (C) 2001 The Hospital infection Society.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 20:04:37