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Titolo:
Studies on the role of the minor capsid protein in transport of Beet western yellows virus through Myzus persicae
Autore:
Reinbold, C; Gildow, FE; Herrbach, E; Ziegler-Graff, V; Goncalves, MC; van den Heuvel, JFJM; Brault, V;
Indirizzi:
INRA, F-68021 Colmar, France INRA Colmar France F-68021INRA, F-68021 Colmar, France Penn State Univ, Dept Plant Pathol, University Pk, PA 16802 USA Penn StateUniv University Pk PA USA 16802 l, University Pk, PA 16802 USA IBMP, F-67084 Strasbourg, France IBMP Strasbourg France F-67084IBMP, F-67084 Strasbourg, France CEIB, Inst Biol, BR-13001970 Campinas, SP, Brazil CEIB Campinas SP BrazilBR-13001970 BCl, BR-13001970 Campinas, SP, Brazil De Ruiter Seeds, NL-2660 BB Bergschenhoek, Netherlands De Ruiter Seeds Bergschenhoek Netherlands NL-2660 BB enhoek, Netherlands
Titolo Testata:
JOURNAL OF GENERAL VIROLOGY
, volume: 82, anno: 2001,
parte:, 8
pagine: 1995 - 2007
SICI:
0022-1317(200108)82:<1995:SOTROT>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
POTATO LEAFROLL VIRUS; APHID SALIVARY-GLAND; READTHROUGH DOMAIN; DWARF LUTEOVIRUSES; MOLECULAR BEACONS; PLANT-VIRUSES; TRANSMISSION; VECTOR; ACQUISITION; INFECTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Brault, V INRA, 28 Rue Herrlisheim, F-68021 Colmar, France INRA 28 Rue Herrlisheim Colmar France F-68021 21 Colmar, France
Citazione:
C. Reinbold et al., "Studies on the role of the minor capsid protein in transport of Beet western yellows virus through Myzus persicae", J GEN VIROL, 82, 2001, pp. 1995-2007

Abstract

Beet western yellows virus (BWYV), family Luteoviridae, is an icosahedral plant virus which is strictly transmitted by aphids in a persistent and circulative manner. Virions cross two cellular barriers in the aphid by receptor-based mechanisms involving endocytosis and exocytosis. Particles are first transported across intestinal cells into the haemolymph and then across accessory salivary gland cells for delivery to the plant via saliva, We identified the midgut part of the digestive tract as the site of intestinal passage by BWYV virions. To analyse the role in transmission of the minor capsid component, the readthrough (RT) protein, the fate of a BWYV RT-deficient non-transmissible mutant was followed by transmission electron microscopyin the vector Myzus persicae. This mutant was observed in the gut lumen but was never found inside midgut cells. However, virion aggregates were detected in the basal lamina of midgut cells when BWYV antiserum was microinjected into the haemolymph. The presence of virions in the haemolymph was confirmed by a sensitive molecular technique for detecting viral RNA. Thus, transport of the mutant virions through intestinal cells occurred but at a lowfrequency. Even when microinjected into the haemolymph, the RT protein mutant was never detected near or in the accessory salivary gland cells. We conclude that the RT protein is not strictly required for the transport of virus particles through midgut cells, but is necessary for the maintenance ofvirions in the haemolymph and their passage through accessory salivary gland cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 12:10:25