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Titolo:
Mapping of QTLs associated with cytosolic glutamine synthetase and NADH-glutamate synthase in rice (Oryza sativa L.)
Autore:
Obara, M; Kajiura, M; Fukuta, Y; Yano, M; Hayashi, M; Yamaya, T; Sato, T;
Indirizzi:
Tohoku Univ, Grad Sch Life Sci, Aoba Ku, Sendai, Miyagi 9808577, Japan Tohoku Univ Sendai Miyagi Japan 9808577 Ku, Sendai, Miyagi 9808577, Japan Tohoku Univ, Grad Sch Agr Sci, Dept Appl Plant Sci, Aoba Ku, Sendai, Miyagi 9818555, Japan Tohoku Univ Sendai Miyagi Japan 9818555 Ku, Sendai, Miyagi 9818555, Japan Int Rice Res Inst, Plant Breeding Genet & Biochem Div, Makati 1271, Philippines Int Rice Res Inst Makati Philippines 1271 Div, Makati 1271, Philippines Natl Inst Agrobiol Resources, Dept Mol Genet, Tsukuba, Ibaraki 3058602, Japan Natl Inst Agrobiol Resources Tsukuba Ibaraki Japan 3058602 3058602, Japan Natl Inst Basic Biol, Dept Cell Biol, Okazaki, Aichi 4448585, Japan Natl Inst Basic Biol Okazaki Aichi Japan 4448585 ki, Aichi 4448585, Japan RIKEN, Plant Sci Ctr, Wako, Saitama 3510198, Japan RIKEN Wako Saitama Japan 3510198 nt Sci Ctr, Wako, Saitama 3510198, Japan
Titolo Testata:
JOURNAL OF EXPERIMENTAL BOTANY
fascicolo: 359, volume: 52, anno: 2001,
pagine: 1209 - 1217
SICI:
0022-0957(200106)52:359<1209:MOQAWC>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELLULAR-LOCALIZATION; MOLECULAR DISSECTION; QUANTITATIVE TRAITS; VASCULAR BUNDLES; LEAF BLADES; PROTEIN; PLANTS; LEAVES; NITROGEN; ENZYME;
Keywords:
cytosolic glutamine synthetase; NADH-glutamate synthase; nitrogen recycling; QTL; rice (Oryza sativa L.);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Sato, T Tohoku Univ, Grad Sch Life Sci, Aoba Ku, 2-1-1 Katahira, Sendai, Miyagi 9808577, Japan Tohoku Univ 2-1-1 Katahira Sendai Miyagi Japan 98085778577, Japan
Citazione:
M. Obara et al., "Mapping of QTLs associated with cytosolic glutamine synthetase and NADH-glutamate synthase in rice (Oryza sativa L.)", J EXP BOT, 52(359), 2001, pp. 1209-1217

Abstract

Ninety-eight backcross inbred lines (BC1F6) developed between Nipponbare, a japonica rice, and Kasalath, an indica rice were employed to detect putative quantitative trait loci (QTLs) associated with the contents of cytosolic glutamine synthetase (GS1; EC 6.3.1.2) and NADH-glutamate synthase (NADH-GOGAT; EC 1.4.1.14) in leaves. Immunoblotting analyses showed transgressivesegregations toward lower or greater contents of these enzyme proteins in these backcross inbred lines. Seven chromosomal QTL regions for GS1 proteincontent and six for NADH-GOGAT protein content were detected. Some of these QTLs were located in QTL regions for various biochemical and physiological traits affected by nitrogen recycling. These findings suggested that the variation in GS1 and NADH-GOGAT protein contents in this population is related to the changes in the rate of nitrogen recycling from senescing organs to developing organs, leading to changes in these physiological traits. Furthermore, a structural gene for GS1 was mapped between two RFLP markers, C560 and C1408, on chromosome 2 and co-located in the QTL region for one-spikelet weight. A QTL region for NADH-GOGAT protein content was detected at the position mapped for the NADH-GOGAT structural gene on chromosome 1. A QTLregion for soluble protein content in developing leaves was also detected in this region. Although fine mapping is required to identify individual genes in the future, QTL analysis could be a useful post-genomic tool to study the gene functions for regulation of nitrogen recycling in rice.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 00:53:55