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Titolo:
Human dendritic cells pulsed with autologous Epstein-Barr virus transformed B-cell lymphoblastoid cell (BCL) lysate elicit a BCL specific MHC-Class II restricted T-cell response
Autore:
Sugano, M; Conway, TF; Kelleher, RJ; Sugiyama, Y; Chen, FA; Bankert, RB; Bernstein, SH;
Indirizzi:
Roswell Pk Canc Inst, Div Med, Buffalo, NY 14263 USA Roswell Pk Canc InstBuffalo NY USA 14263 Div Med, Buffalo, NY 14263 USA
Titolo Testata:
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
fascicolo: 2, volume: 20, anno: 2001,
pagine: 175 - 182
SICI:
0392-9078(200106)20:2<175:HDCPWA>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
MELANOMA ANTIGENS; APOPTOTIC CELLS; TUMOR-ANTIGENS; HUMAN BLOOD; LYMPHOCYTES; RECOGNIZE; PROGENITORS; GENERATION; CYTOKINES; IMMUNITY;
Keywords:
Epstein-Barr virus; cell lysate; dendritic cells; cytotoxic T-cells; MHC class II;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Bernstein, SH Roswell Pk Canc Inst, Div Med, Elm & Carlton St, Buffalo, NY14263 USA Roswell Pk Canc Inst Elm & Carlton St Buffalo NY USA 14263 A
Citazione:
M. Sugano et al., "Human dendritic cells pulsed with autologous Epstein-Barr virus transformed B-cell lymphoblastoid cell (BCL) lysate elicit a BCL specific MHC-Class II restricted T-cell response", J EXP CL C, 20(2), 2001, pp. 175-182

Abstract

Epstein Barr Virus (EBV) associated lymphoproliferative disorders (LPD) express EBV latent antigens that are also expressed on normal B-cells transformed with EBV. This could potentially be exploited to develop immunotherapeutic strategies for LPD and other EBV associated malignancies. To this end we investigated the capacity of human monocyte derived dendritic cells (DC)pulsed with lysate from autologous EBV transformed B-cell lymphoblastoid cell (BCL) lysate to elicit an in vitro antitumor response. BCL lysate pulsed DC generate BCL specific cytotoxic lymphocytes, as lymphocytes primed with such DCs induce cytolysis of autologous (> 60%) but not allogeneic BCL(<5%). In addition, lymphocytes primed with BCL lysate pulsed DC secrete gamma-IFN (3176 pg/ml). Whereas gamma -IFN production was markedly reduced (> 99%) when BCL specific T-cells were stimulated by BCL lysate pulsed DC in the presence of blocking antibodies to HLA-DR, DP and DQ, use of antibodies to MHC class-I resulted in only a minimal reduction in gamma -IFN production(17%). These studies demonstrate that BCL lysate pulsed DC elicit a predominantly BCL specific, MHC class-II restricted T cell response. This suggeststhat vaccination with autologous BCL lysate pulsed DC may represent a viable immunotherapeutic approach for the treatment of LPD.

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Documento generato il 01/10/20 alle ore 16:13:38