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Titolo:
Influence of acyl chain fluidity on the lipopolysaccharide-induced activation of complement
Autore:
Wiese, A; Grunewald, P; Schaper, KJ; Seydel, U;
Indirizzi:
Res Ctr Borstel, Ctr Med & Biosci, Div Biophys, D-23845 Borstel, Germany Res Ctr Borstel Borstel Germany D-23845 iophys, D-23845 Borstel, Germany
Titolo Testata:
JOURNAL OF ENDOTOXIN RESEARCH
fascicolo: 2, volume: 7, anno: 2001,
pagine: 147 - 155
SICI:
0968-0519(2001)7:2<147:IOACFO>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTIBODY-INDEPENDENT ACTIVATION; GRAM-NEGATIVE BACTERIA; HUMAN-SERUM COMPLEMENT; LIPID-A; OUTER-MEMBRANE; ESCHERICHIA-COLI; INFRARED-SPECTROSCOPY; ALTERNATIVE PATHWAYS; BIOLOGICAL-ACTIVITY; SURFACE-CHARGE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Seydel, U Res Ctr Borstel, Ctr Med & Biosci, Div Biophys, Pk Allee 10, D-23845 Borstel, Germany Res Ctr Borstel Pk Allee 10 Borstel Germany D-23845 el, Germany
Citazione:
A. Wiese et al., "Influence of acyl chain fluidity on the lipopolysaccharide-induced activation of complement", J ENDOTOX R, 7(2), 2001, pp. 147-155

Abstract

Lipopolysaccharides (LPSs. endotoxins) are the major amphiphilic constituents of the outer leaflet of the outer membrane of Gram-negative bacteria. They are known to activate the complement cascade to form lytic membrane pores. Here, we study the influence of the fluidity of the acyl chains of LPSsand lipid As on the formation of lytic pores. To this end, we have performed electrical measurements on asymmetric planar endotoxin/phospholipid bilayers as a reconstitution model of the outer membrane using two deep rough mutant LPSs (from Escherichia coli strains WBB01 and WBB25) and two lipid As(from E. coli WBB25 and Rhodobacter sphaeroides). The two LPSs and the twolipid As each differ in their acylation pattern which is correlated with the fluidity. The addition of human serum to the endotoxin side of the bilayers led to the formation of membrane pores, and pore formation correlated in each case with acyl chain fluidity. i.e. time required for the first lytic pore to be formed was shorter for the more fluid endotoxin. Furthermore, in the case of LPSs, the activation rate was higher for the more fluid membrane and the respective bacteria had a higher susceptibility to the growth inhibitory action of serum.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/04/20 alle ore 15:58:49