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Titolo:
Dynamics of viral quasispecies during interferon therapy in non responder chronic hepatitis C patients
Autore:
Thelu, MA; Baud, M; Leroy, V; Seigneurin, JM; Zarski, JP;
Indirizzi:
Hop Albert Michallon, Dept Hepatogastroenterol, F-38043 Grenoble, France Hop Albert Michallon Grenoble France F-38043 l, F-38043 Grenoble, France Fac Med, Lab Virol Struct & Mol, F-38706 La Tronche, France Fac Med La Tronche France F-38706 ruct & Mol, F-38706 La Tronche, France
Titolo Testata:
JOURNAL OF CLINICAL VIROLOGY
fascicolo: 1, volume: 22, anno: 2001,
pagine: 125 - 131
SICI:
1386-6532(200108)22:1<125:DOVQDI>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
STRAND CONFORMATION POLYMORPHISM; HYPERVARIABLE REGION-1; VIRUS HCV; GENOTYPE 1B; 5A GENE; INFECTION; MUTATIONS; COMPLEXITY; PROTEIN; RNA;
Keywords:
chronic hepatitis C virus; interferon; HCV RNA; heterogeneity; quasispecies; SSCP analysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Thelu, MA Hop Albert Michallon, Dept Hepatogastroenterol, F-38043 Grenoble, France Hop Albert Michallon Grenoble France F-38043 Grenoble, France
Citazione:
M.A. Thelu et al., "Dynamics of viral quasispecies during interferon therapy in non responder chronic hepatitis C patients", J CLIN VIRO, 22(1), 2001, pp. 125-131

Abstract

Background: the reference method to study the HCV complexity was cloning and sequence analysis of a sufficient number of clones. The evolution of theviral complexity in chronic non responder patients during treatment with standard doses of interferon was not very well investigate because this method was expensive and labour intensive when large series of patients were concerned. Meanwhile, with the alternative Single-Strand Conformation Polymorphism (SSCP) method, a rough estimation of the quasispecies present in a given sample could be obtained. Objectives: the aim of the study was to analyse the evolution of HCV heterogeneity, investigated by SSCP analysis targeted to the HVR-1, in 30 nonresponders chronic hepatitis C patients treated by Interferon-alpha 3MUI. Results: genotype 1 was the main HCV type found inthis population (77% of non responder patients). Before treatment, the SSCP assay revealed a high complexity pattern: the median of SSCP band number was 9. During IFN-a treatment, SSCP band number didn't change. However a significant decrease of the viral load was observed (P < 0.01). Patients withvariations in their SSCP patterns after therapy significantly decreased HCV RNA levels (P < 0.002). In one third of patients the SSCP profile didn't change at all. Conclusions: we observed that viral heterogeneity didn't change in non responder chronic hepatitis C patients during IFN-cl treatment. Nevertheless patients with a low number of pre-treatment quasispecies exhibited an improvement of the response (P < 0.02). These phenomena were probably due to a selection of resistant variants present prior onset of therapy. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 16:13:54