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Titolo:
Comparison of systems performance for TT virus detection using PCR primer sets located in non-coding and coding regions of the viral genome
Autore:
Biagini, P; Gallian, P; Attoui, H; Cantaloube, JF; Touinssi, M; de Micco, P; de Lamballerie, X;
Indirizzi:
Fac Med Marseille, Lab Virol Mol Trop & Transfus, Unite Virus Emergents, F-13005 Marseille, France Fac Med Marseille Marseille France F-13005 ts, F-13005 Marseille, France Estab Francais Sang Alpes Mediterranee, Mol Virol Lab, Unite Virus Emergents, F-13005 Marseille, France Estab Francais Sang Alpes Mediterranee Marseille France F-13005 , France
Titolo Testata:
JOURNAL OF CLINICAL VIROLOGY
fascicolo: 1, volume: 22, anno: 2001,
pagine: 91 - 99
SICI:
1386-6532(200108)22:1<91:COSPFT>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTRAVENOUS-DRUG-USERS; FRENCH BLOOD-DONORS; HIGH PREVALENCE; NONHUMAN-PRIMATES; PHYLOGENETIC ANALYSIS; HUMAN CIRCOVIRUS; DNA GENOME; INFECTION; SEQUENCES; IDENTIFICATION;
Keywords:
TT virus; molecular diagnosis; polymerase chain reaction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: de Lamballerie, X Fac Med Marseille, Lab Virol Mol Trop & Transfus, Unite Virus Emergents, 27 Bd J Moulin, F-13005 Marseille, France Fac Med Marseille 27 Bd J Moulin Marseille France F-13005
Citazione:
P. Biagini et al., "Comparison of systems performance for TT virus detection using PCR primer sets located in non-coding and coding regions of the viral genome", J CLIN VIRO, 22(1), 2001, pp. 91-99

Abstract

Background: the heterogeneity of the TT virus (TTV) DNA prevalence values reported from comparable human cohorts suggests that diagnostic PCR protocols still require to be optimized. Objectives: to design TTV PCR primer setswith low genotype restriction and to compare their performances with commonly used amplification systems. Study design: we compared full length TTV genomic sequences and identified conserved nucleotide patterns in the 5' and3' non-coding regions of the viral genome. This permitted to design two new primer sets usable for the PCR amplification of the most divergent human isolates of TTV described to date. The performances of these amplification systems were compared with those of three other PCR systems earlier used for prevalence studies. Results: the primer systems P5Bx and P3Bx exhibited higher PCR scores than the other systems tested; 14 to 34% improvement values were obtained, and divergent positive results of earlier described PCR systems were confirmed systematically by our new detection assays. Conclusions: an optimized detection of TT virus DNA is a pre-requisite for the accurate epidemiological survey of viral infection and for the realization of phylogenetic studies. Such PCR systems with low genotype restriction will be helpful in the future for a better knowledge of natural history of TT virus infection. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 20:10:22