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Titolo:
From fixed to FRAP: measuring protein mobility and activity in living cells
Autore:
Reits, EAJ; Neefjes, JJ;
Indirizzi:
Netherlands Canc Inst, Div Tumor Biol, NL-1066 CX Amsterdam, Netherlands Netherlands Canc Inst Amsterdam Netherlands NL-1066 CX rdam, Netherlands
Titolo Testata:
NATURE CELL BIOLOGY
fascicolo: 6, volume: 3, anno: 2001,
pagine: E145 - E147
SICI:
1465-7392(200106)3:6<E145:FFTFMP>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLUORESCENCE PHOTOBLEACHING RECOVERY; CULTURED MUSCLE-CELLS; LATERAL MOBILITY; MEMBRANE-PROTEIN; TRANSLATIONAL DIFFUSION; ENDOPLASMIC-RETICULUM; DYNAMICS; DEPENDENCE; RETENTION; MOTION;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Reits, EAJ Netherlands Canc Inst, Div Tumor Biol, Plesmanlaan 121, NL-1066CX Amsterdam, Netherlands Netherlands Canc Inst Plesmanlaan 121 AmsterdamNetherlands NL-1066 CX
Citazione:
E.A.J. Reits e J.J. Neefjes, "From fixed to FRAP: measuring protein mobility and activity in living cells", NAT CELL BI, 3(6), 2001, pp. E145-E147

Abstract

Experiments with fluorescence recovery after photobleaching (FRAP) started30 years ago to visualize the lateral mobility and dynamics of fluorescentproteins in living cells. Its popularity increased when non-invasive fluorescent tagging became possible with the green fluorescent protein (GFP). Many researchers use GFP to study the localization of fusion proteins in fixed or living cells, but the same fluorescent proteins can also be used to study protein mobility in living cells. Here we review the potential of FRAP to study protein dynamics and activity within a single living cell. These measurements can be made with most standard confocal laser-scanning microscopes equipped with photobleaching protocols.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 03:20:20