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Titolo:
Partial purification and some biochemical charactcristics of exocellular keratinase from Trichophyton mentagrophytes var. erinacei
Autore:
Muhsin, TM; Aubaid, AH;
Indirizzi:
Basrah Univ, Coll Educ, Dept Biol, Basrah, Iraq Basrah Univ Basrah IraqBasrah Univ, Coll Educ, Dept Biol, Basrah, Iraq
Titolo Testata:
MYCOPATHOLOGIA
fascicolo: 3, volume: 150, anno: 2001,
pagine: 121 - 125
SICI:
0301-486X(2001)150:3<121:PPASBC>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
DERMATOPHYTES; RUBRUM; EXPRESSION; PROTEINASE; CULTURES;
Keywords:
biochemical characteristics; dermatophytes; enzyme purification; keratinase; Trichophyton;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Muhsin, TM Univ Alberta, Dept Renewable Resources, 4-42 Earth Sci Bldg, Edmonton, AB T6G 2E3, Canada Univ Alberta 4-42 Earth Sci Bldg Edmonton AB Canada T6G 2E3 ada
Citazione:
T.M. Muhsin e A.H. Aubaid, "Partial purification and some biochemical charactcristics of exocellular keratinase from Trichophyton mentagrophytes var. erinacei", MYCOPATHOLO, 150(3), 2001, pp. 121-125

Abstract

The dermatophyte Trichophyton mentagrophytes var. erinacei isolated from patients infected with tinea cruris was cultured in Sabouraud dextrose broth, from which an exocellular kenitinase extract was obtained. The keratinasewas partially purified with sephadix G-100 gel filtration. Some biochemical characteristics of the purified enzyme were examined. Its molecular weight was estimated to be 38000 dalton on sodium dodecyle sulfate polyacrylmidegel electrophoresis (SDS-PAGE). The optimal pH was 5.5 and optimal temperature for the highest keratinase activity was 50 degreesC. The enzyme activity was specifically increased against guinea pig hair and fibrous protein and inhibited by phenylmethylsulfonylfloride.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 10:58:12