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Titolo:
Biological X-ray microanalysis: The past, present practices, and future prospects
Autore:
Echlin, P;
Indirizzi:
Univ Cambridge, Sch Biol Sci, MultiImaging Ctr, Cambridge CB4 3DY, EnglandUniv Cambridge Cambridge England CB4 3DY Ctr, Cambridge CB4 3DY, England
Titolo Testata:
MICROSCOPY AND MICROANALYSIS
fascicolo: 2, volume: 7, anno: 2001,
pagine: 211 - 219
SICI:
1431-9276(200103/04)7:2<211:BXMTPP>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Keywords:
biological samples; X-ray microanalysis; low temperatures; sample preparation; analytical procedures; low voltage;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Echlin, P Cambridge Analyt Microscopy, 65 Milton Rd, Cambridge CB4 1XA, England Cambridge Analyt Microscopy 65 Milton Rd Cambridge England CB4 1XA
Citazione:
P. Echlin, "Biological X-ray microanalysis: The past, present practices, and future prospects", MICROS MICR, 7(2), 2001, pp. 211-219

Abstract

A brief description is given of the events surrounding the development of biological X-ray microanalysis during the last 30 years, with particular emphasis on the contribution made by research workers in Cambridge, UK. Therethen follows a broad review of some applications of biological X-ray microanalysis. A more detailed consideration is given to the main thrust of current procedures and applications that are, for convenience, considered as four different kinds of samples. Thin frozen dried sections which are analyzed at ambient temperatures in a transmission electron microscope (TEM); semithin frozen dried sections which are analyzed at low temperature in a scanning transmission electron microscope (STEM); thick frozen hydrated sectionswhich are analyzed at low temperature in a scanning electron microscope (SEM), and bulk samples which are analyzed at low temperature in the same type of instrument. A brief outline is given of the advantages and disadvantages of performing low-voltage, low-temperature X-ray microanalysis on frozenhydrated bulk biological material. The article concludes with a consideration of alternative approaches to in situ analysis using either high-energy beams or visible and near-visible photons.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 22:14:37