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Titolo:
A novel glycosylated Cu/Zn-containing superoxide dismutase: production andpotential therapeutic effect
Autore:
Angelova, M; Dolashka-Angelova, P; Ivanova, E; Serkedjieva, J; Slokoska, L; Pashova, S; Toshkova, R; Vassilev, S; Simeonov, I; Hartmann, HJ; Stoeva, S; Weser, U; Voelter, W;
Indirizzi:
Bulgarian Acad Sci, Inst Microbiol, BU-1113 Sofia, Bulgaria Bulgarian AcadSci Sofia Bulgaria BU-1113 obiol, BU-1113 Sofia, Bulgaria Univ Tubingen, Phys Biochem Abt, Inst Physiol Chem, D-72076 Tubingen, Germany Univ Tubingen Tubingen Germany D-72076 l Chem, D-72076 Tubingen, Germany Bulgarian Acad Sci, Inst Organ Chem, BU-1113 Sofia, Bulgaria Bulgarian Acad Sci Sofia Bulgaria BU-1113 Chem, BU-1113 Sofia, Bulgaria Bulgarian Acad Sci, Inst Expt Pathol & Parasitol, BU-1113 Sofia, Bulgaria Bulgarian Acad Sci Sofia Bulgaria BU-1113 sitol, BU-1113 Sofia, Bulgaria
Titolo Testata:
MICROBIOLOGY-SGM
, volume: 147, anno: 2001,
parte:, 6
pagine: 1641 - 1650
SICI:
1350-0872(200106)147:<1641:ANGCSD>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
SACCHAROMYCES-CEREVISIAE; OXYGEN RADICALS; CANCER-THERAPY; INFLUENZA; ANTIOXIDANTS; INJURY; RATS; SOD; PATHOGENESIS; GENERATION;
Keywords:
protein sequencing; myeloid Graffi tumour; influenza infection; protective effect;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Voelter, W Univ Tubingen, Phys Biochem Abt, Inst Physiol Chem, Hoppe Seyler Str 4, D-72076 Tubingen, Germany Univ Tubingen Hoppe Seyler Str 4 Tubingen Germany D-72076 many
Citazione:
M. Angelova et al., "A novel glycosylated Cu/Zn-containing superoxide dismutase: production andpotential therapeutic effect", MICROBI-SGM, 147, 2001, pp. 1641-1650

Abstract

The fungal strain Humicola lutea 103 produces a naturally glycosylated Cu/Zn SOD, To improve its yield, the effect of an increased concentration of dissolved oxygen (DO) on growth and enzyme biosynthesis by the producer, cultivated in a 3I bioreactor, was examined. Exposure to a 20% DO level causeda 1.7-fold increase of SOD activity compared to the DO-uncontrolled culture. Maximum enzyme productivity of SOD was approximately 300 x 10(3) U (kg wet biomass)(-1) The novel enzyme was purified to electrophoretic homogeneity, The presence of Cu and Zn were confirmed by atomic absorption spectrometry, The molecular mass of H. lutes Cu/Zn SOD was calculated to be 31870 Da for the whole molecule and 15936 Da for the structural subunits, The N-terminal sequence revealed a high degree of structural homology with Cu/Zn SOD from other prokaryotic and eukaryotic sources, if, lutea Cu/Zn SOD was usedin an in vivo model for the demonstration of its protective effect againstmyeloid Graffi tumour in hamsters, Comparative studies revealed that the enzyme (i) elongated the latent time for tumour appearance, (ii) inhibited tumour growth in the early stage of tumour progression (73-75% at day 10) and (iii) increased the mean survival time of Graffi-tumour-bearing hamsters,Moreover, the fungal Cu/Zn SOD exhibited a strong protective effect on experimental influenza virus infection in mice. The survival rate increased markedly, the H. lutea SOD protected mice from mortality more efficiently compared to me selective antiviral drug ribavirin and to commercial bovine SOD, In conclusion, our results suggest that appropriate use of the novel fungal SOD. applied as time of survival rose by 5.2 d and the protective index reached 86%, The H. lutea SOD protected mice from mortality more efficiently compared to the such or in combination with selective inhibitors, could outline a promising strategy for the treatment of myeloid Graffi tumour and influenza virus infection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 03:30:12