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Titolo:
Thymic lesions in cats infected with a pathogenic molecular clone or an ORF-A/2-deficient molecular clone of feline immunodeficiency virus
Autore:
Norway, RM; Crawford, PC; Johnson, CM; Mergia, A;
Indirizzi:
Univ Florida, Dept Pathobiol, Coll Vet Med, Gainesville, FL 32610 USA UnivFlorida Gainesville FL USA 32610 Vet Med, Gainesville, FL 32610 USA
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 13, volume: 75, anno: 2001,
pagine: 5833 - 5841
SICI:
0022-538X(200107)75:13<5833:TLICIW>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG TERMINAL REPEAT; SCID-HU MICE; LYMPHOID-TISSUES; JUVENILE CATS; VIRAL REPLICATION; INTERFERON-GAMMA; ACUTE STAGE; IDENTIFICATION; LYMPHOCYTES; TROPISM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Mergia, A Univ Florida, Dept Pathobiol, Coll Vet Med, POB 110880, Gainesville, FL 32610 USA Univ Florida POB 110880 Gainesville FL USA 32610 e, FL 32610 USA
Citazione:
R.M. Norway et al., "Thymic lesions in cats infected with a pathogenic molecular clone or an ORF-A/2-deficient molecular clone of feline immunodeficiency virus", J VIROLOGY, 75(13), 2001, pp. 5833-5841

Abstract

Previous studies using feline immunodeficiency virus (FIV) molecular clones lacking the putative transactivator gene (ORF-A/2) failed to address the issue of thymus pathogenesis or investigate the levels of viral replicationin separate lymphoid compartments (Y, Inoshima, et al,, J, Virol, 70:8518-8526, 1996; E, E, Sparger, et al,, Virology 205:546-553, 1994), Using a highly pathogenic molecular clone of FIV, JSY3, and an ORF-A/2-deficient mutant, JSY3 Delta ORF-A/Z, we compared viral replication and the extent of thymic dysfunction as measured by the formation of lymphoid follicles and alteration of the thymocyte subsets. Viral replication was reduced in JSY3 DeltaORF-A/2-infected cats as measured by lymphocyte coculture, immunohistochemistry, and quantitative PCR, Cell-associated viral load measured by lymphocyte coculture varied in a tissue-dependent manner with replication highest in lymphocytes isolated from the thymus, lower in those from the peripheralblood, and lowest in those from lymph node. Thymic proviral load and the number of viral p24 Gag-positive cells within the thymus detected by immunohistochemistry were also reduced. In addition, the onset of a reduced peripheral blood CD4/CD8 ratio was delayed in JSY3 Delta ORF-A/2-infected cats. The formation and extent of thymic lymphoid follicular hyperplasia were similar in JSY3 and JSY3 Delta ORF-A/2-infected cats as measured by anticytokeratin immunohistochemistry and flow cytometry for percent pan T-negative, immunoglobulin G-positive cells within the thymus. Tn contrast, comparison ofthymocyte subpopulations demonstrated a reduced expansion of single-positive CD4(-) CD8(+) thymocytes in JSY3 Delta ORF-A/2-infected cats. Level of viral replication, therefore, may not correlate with the formation of thymiclymphoid follicles but may correlate with the expansion of the single-positive CD4(-) CD8(+) thymocyte subpopulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 05:34:15