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Titolo:
Vascular endothelial growth factor promotes proliferation and migration ofcavernous smooth muscle cells
Autore:
Liu, XW; Lin, CS; Graziottin, T; Resplande, J; Lue, TF;
Indirizzi:
Univ Calif San Francisco, Sch Med, Dept Urol, Knuppe Mol Urol Lab, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA
Titolo Testata:
JOURNAL OF UROLOGY
fascicolo: 1, volume: 166, anno: 2001,
pagine: 354 - 360
SICI:
0022-5347(200107)166:1<354:VEGFPP>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR EXPRESSION; VEGF-D; RECEPTORS; PLACENTA; GENE; IDENTIFICATION; RATS; KDR;
Keywords:
muscle, smooth; penis; rats, Wistar; endothelial growth factors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Liu, XW Univ Calif San Francisco, Sch Med, Dept Urol, Knuppe Mol Urol Lab,San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA94143 CA 94143 USA
Citazione:
X.W. Liu et al., "Vascular endothelial growth factor promotes proliferation and migration ofcavernous smooth muscle cells", J UROL, 166(1), 2001, pp. 354-360

Abstract

Purpose: We investigated whether cavernous smooth muscle cells secrete vascular endothelial growth factor (VEGF), how they respond to VEGF and how age affects their ability to secrete and respond to VEGF. Materials and Methods: Corpus cavernous tissues were isolated from rats ofvarious ages and grown as monolayer cell cultures. Smooth muscle identity was determined by immunofluorescence staining. Secreted VEGF was measured with a VEGF enzyme-linked immunosorbent assay kit. Cell proliferation was assayed with a cell titer kit. Cell migration was measured with Transwell chambers. VEGF receptor (VEGFR) messenger RNA and proteins were identified by reverse transcription-polymerase chain reaction and immunoblotting, respectively. Results: Cavernous smooth muscle cells from young (age 1 week) and old (age 28 months) rats secreted the least; amounts of VEGF and those from 16-week-old rats secreted the most;. VEGF stimulated the growth of cavernous smooth muscle cells at an optimal concentration of 12.5 ng./ml. At this concentration cavernous smooth muscle cells from 11-week-old and 28-month-old ratsshowed the highest and lowest growth rates, respectively. The optimal VEGFconcentration for cavernous smooth muscle cell migration was 10 ng./ml. Atthis concentration cells from 4-week-old and 28-month-old rats showed the highest and lowest migratory rates, respectively. The expression of VEGFR-1appeared to parallel the VEGF regulated growth rates of these cells. VEGFR-2 was undetectable in cavernous smooth muscle cells. Conclusions: Cavernous smooth muscle cells from rats of different ages secreted different amounts of VEGF and showed different abilities to respond to VEGF. Cavernous smooth muscle cells responded to VEGF in terms of cell proliferation and migration. Cavernous smooth muscle cells from rats of different ages expressed different levels of VEGFR-1 but did not express VEGFR-2.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 00:25:47