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Titolo:
Characterization of the spontaneous electrical and contractile activity ofsmooth muscle cells in the rat upper urinary tract
Autore:
Lang, RJ; Takano, H; Davidson, ME; Suzuki, H; Klemm, MF;
Indirizzi:
Monash Univ, Dept Physiol, Clayton, Vic 3168, Australia Monash Univ Clayton Vic Australia 3168 siol, Clayton, Vic 3168, Australia Nagoya City Univ, Dept Physiol, Nagoya, Aichi 467, Japan Nagoya City UnivNagoya Aichi Japan 467 Physiol, Nagoya, Aichi 467, Japan
Titolo Testata:
JOURNAL OF UROLOGY
fascicolo: 1, volume: 166, anno: 2001,
pagine: 329 - 334
SICI:
0022-5347(200107)166:1<329:COTSEA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-RELATED PEPTIDE; GUINEA-PIG; RENAL PELVIS; PACEMAKER ACTIVITY; MODULATION; NEURONS; SYSTEM;
Keywords:
peristalsis; action potentials; rats, Wistar; urinary tract; muscle, smooth;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Lang, RJ Monash Univ, Dept Physiol, Clayton, Vic 3168, Australia Monash Univ Clayton Vic Australia 3168 yton, Vic 3168, Australia
Citazione:
R.J. Lang et al., "Characterization of the spontaneous electrical and contractile activity ofsmooth muscle cells in the rat upper urinary tract", J UROL, 166(1), 2001, pp. 329-334

Abstract

Purpose: We morphologically and electrophysiologically identified the cells that generate the electrical activity underlying the peristaltic contractions of the rat upper urinary tract. Materials and Methods: Electron microscopy and tension recording techniques were used to characterize the smooth muscle cells underlying spontaneous contractions in the wall of the rat ureter, and proximal and distal renal pelvis. Intracellular microelectrodes, containing 4% neurobiotin were used to record data from the cells of the renal pelvis, which were later viewed on a confocal microscope. Results: Spontaneous myogenic contractions (average 22.3 +/- 2.2 minutes(-1)) originated in the proximal renal pelvis and propagated into the distal renal pelvis and ureter in 6 preparations. Smooth muscle cells in the renalpelvis and ureter were typical in appearance with greater than 85% of their sectional area containing clumped contractile filaments. In contrast, contractile fibrils occupied only 65% of the sectional area of the smooth muscle cells within the most proximal region of the renal pelvis (pelvicalicealjunction). In strips of the renal pelvis spindle shaped cells 83 to 200 mum. long fired spontaneous action potentials (6 minutes(-1)) consisting of an initial spike, a quiescent plateau phase and abrupt hyperpolarization to a peak diastolic potential of -60 mV. Other spindle shaped cells 94 to 112 mum. long displayed small membrane transients (15 minutes(-1)) 9 to 19 mV. in amplitude, firing from a diastolic potential of -40 mV. Conclusions: It is likely that the spontaneous contractile activity of therat upper urinary tract arises from the discharge of action potentials in typical smooth muscle cells of the proximal renal pelvis that are directly driven by the spontaneous membrane oscillations of atypical smooth muscle cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 13:25:12