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Titolo:
EFFECTS OF HIV-1 ON THE SURFACE EXPRESSION OF LFA-1 ON CULTURED MONOCYTES
Autore:
STENT G; CROWE SM;
Indirizzi:
MACFARLANE BURNET CTR MED RES,NATL CTR HIV VIROL RES,AIDS PATHOGENESIS RES UNIT,POB 254 FAIRFIELD VIC 3078 AUSTRALIA MACFARLANE BURNET CTR MED RES,NATL CTR HIV VIROL RES,AIDS PATHOGENESIS RES UNIT FAIRFIELD VIC 3078 AUSTRALIA
Titolo Testata:
Journal of acquired immune deficiency syndromes and human retrovirology
fascicolo: 2, volume: 15, anno: 1997,
pagine: 95 - 103
SICI:
1077-9450(1997)15:2<95:EOHOTS>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; LEUKOCYTE ADHESION MOLECULES; INDUCED SYNCYTIUM FORMATION; MONONUCLEAR PHAGOCYTES; CELLS INVITRO; CLASS-II; T-CELLS; GM-CSF; INFECTION; MACROPHAGES;
Keywords:
LFA-1; MONOCYTES; HIV-1; RECOMBINANT SOLUBLE CD4; AZT; RECOMBINANT GP120; GRANULOCYTOMACROPHAGE COLONY-STIMULATING FACTOR; ZIDOVUDINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
G. Stent e S.M. Crowe, "EFFECTS OF HIV-1 ON THE SURFACE EXPRESSION OF LFA-1 ON CULTURED MONOCYTES", Journal of acquired immune deficiency syndromes and human retrovirology, 15(2), 1997, pp. 95-103

Abstract

CD11a, the alpha chain of LFA-1, which is a member of the LeuCAM family of integrins, has been implicated in the formation of HIV-induced syncytia and may contribute to the depletion of CD4-positive lymphocytes seen in patients with HIV infection. In this study, we examined the effects of HIV-1 infection on the expression of CD11a on cultured monocyte-derived macrophages (MDMs). Monocytes isolated from peripheral blood and maintained in suspension culture were infected in vitro with amonocytotropic variant of HIV-1 (Ba-L). Surface expression of CD11a, measured by indirect immunofluorescence and flow cytometry, was significantly higher on HIV-infected cells than on mock-infected cells from the same donor. Upregulation of CD11a expression was unaffected by theHIV reverse transcriptase inhibitor, zidovudine, indicating that it did not depend on reverse transcription. A step before reverse transcription, such as viral binding, appears sufficient to trigger an increase in CD11a expression. This hypothesis is supported by our findings ofsoluble recombinant CD4 inhibition of HIV-induced CD11a upregulation. It is possible that induction of a cytokine network by HIV underlies this effect, given our findings that exposure of uninfected MDMs to granulocyte-macrophage colony-stimulating factor (GM-CSF) specifically increased CD11a expression and that HIV-infected MDMs secreted more GM-CSF than mock-infected cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 16:45:04