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Titolo:
Caspase-3 inhibition partially protects oxidant production in apoptotic human neutrophils
Autore:
Sweeney, JF; Nguyen, PK; Hinshaw, DB;
Indirizzi:
Univ Michigan, Dept Surg, Ann Arbor, MI 48109 USA Univ Michigan Ann ArborMI USA 48109 , Dept Surg, Ann Arbor, MI 48109 USA Ann Arbor VA Med Ctr, Surg Serv, Ann Arbor, MI 48109 USA Ann Arbor VA Med Ctr Ann Arbor MI USA 48109 Serv, Ann Arbor, MI 48109 USA
Titolo Testata:
JOURNAL OF SURGICAL RESEARCH
fascicolo: 1, volume: 98, anno: 2001,
pagine: 66 - 70
SICI:
0022-4804(20010601)98:1<66:CIPPOP>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
FC-GAMMA-RIII; POLYMORPHONUCLEAR LEUKOCYTES; AGING NEUTROPHILS; CELL-DEATH; INFLAMMATION; RECOGNITION; PHAGOCYTOSIS; MACROPHAGES; RESOLUTION; ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Sweeney, JF 2920G Taubman ctr,1500 E Med Ctr Dr, Ann Arbor, MI 48109 USA 2920G Taubman ctr,1500 E Med Ctr Dr Ann Arbor MI USA 48109 SA
Citazione:
J.F. Sweeney et al., "Caspase-3 inhibition partially protects oxidant production in apoptotic human neutrophils", J SURG RES, 98(1), 2001, pp. 66-70

Abstract

Apoptotic PMN lose functional activity, which emphasizes the tissue injurylimiting potential of PMN apoptosis, Caspase-3 activation is the first step in the execution phase of apoptosis. We hypothesized that PMN functional activity, as evidenced by oxidant production, can be restored in apoptotic PMN by inhibition of caspase-3,Methods. To accelerate PMN apoptosis, PMN were W-irradiated for 15 min as previously described. PMN were pretreated with the caspase-3 inhibitor DEVD-fmk (100 muM) for 30 min prior to UV, PMN apoptosis was quantitated by flow cytometry with CD16 staining. Oxidant production in response to 10 muM PMA was quantitated fluorometrically using the method of Hyslop and Sklar, Caspase-3 activity was quantitated fluorometrically using a commercially available assay,Results. UV-treated PMN demonstrated a 3-fold increase in caspase-3 activity, This was associated with a significant increase in apoptotic PMN and a 10-fold decrease in oxidant production compared to control PMN. DEVD-fmk blocked increases in caspase-3 activity and significantly reduced PMN apoptosis, Oxidant production was increased 5-fold compared to UV-treated PMN but was still significantly less than control PMN. Conclusions. In UV-accelerated PMN apoptosis, inhibition of caspase-3 activity partially protects oxidant production in apoptotic PMN, This suggests that signaling events in the initiation phase of PMN apoptosis, which are proximal to caspase-3 activation, may in part be responsible for loss of oxidant production in apoptotic PMN independent of caspase-3 activity. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 07:25:26