Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Role of ceramide during cisplatin-induced apoptosis in C6 glioma cells
Autore:
Noda, S; Yoshimura, SI; Sawada, M; Naganawa, T; Iwama, T; Nakashima, S; Sakai, N;
Indirizzi:
Gifu Univ, Sch Med, Dept Neurosurg, Gifu 5008705, Japan Gifu Univ Gifu Japan 5008705 ch Med, Dept Neurosurg, Gifu 5008705, Japan Gifu Univ, Sch Med, Dept Biochem, Gifu 5008705, Japan Gifu Univ Gifu Japan 5008705 Sch Med, Dept Biochem, Gifu 5008705, Japan
Titolo Testata:
JOURNAL OF NEURO-ONCOLOGY
fascicolo: 1, volume: 52, anno: 2001,
pagine: 11 - 21
SICI:
0167-594X(200103)52:1<11:ROCDCA>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN GLIOBLASTOMA CELLS; NEUTRAL SPHINGOMYELINASE; CASPASE-3 ACTIVATION; COMBINATION THERAPY; ICE/CED-3 PROTEASE; MEDIATED APOPTOSIS; PHOSPHOLIPASE-D; CYTOCHROME-C; GLIAL-CELLS; DEATH;
Keywords:
apoptosis; antioxidant; caspase; cisplatin; glioma; signal transduction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Noda, S Gifu Univ, Sch Med, Dept Neurosurg, Tsukasamachi 40, Gifu 5008705,Japan Gifu Univ Tsukasamachi 40 Gifu Japan 5008705 Gifu 5008705, Japan
Citazione:
S. Noda et al., "Role of ceramide during cisplatin-induced apoptosis in C6 glioma cells", J NEURO-ONC, 52(1), 2001, pp. 11-21

Abstract

Cisplatin is commonly used for the treatment of malignant brain tumors. However, the mechanisms of cell death by cisplatin are not fully understood. Therefore, the present study was designed to elucidate the apoptotic signaling pathway(s) activated by cisplatin in a C6 rat glioma cell line. C6 cells were treated with various concentrations of cisplatin (0.2-10 mug/ml) for24-72 h. At 10 mug/ml cisplatin, over 90% of the cells became dead at 72 h. Apoptotic death was confirmed by condensation and fragmentation of nuclei, and DNA laddering. Even in cells treated with 1.5 mug/ml cisplatin, typical apoptotic cells were observed at 72 h. The intracellular level of ceramide, measured Escherichia coli diacylglycerol kinase markedly increased during 24-72 h after the addition of 10 mug/ml cisplatin. The activity of caspase-3(-like) proteases increased and reached a peak at 48 h. Inhibitors of caspases reduced the number of apoptotic cells. Pretreatment of C6 cells with glutathione or N-acetyl-cysteine, which are known to block the activationof neutral magnesium-dependent sphingomyelinase, inhibited ceramide formation, leading to suppression of both activation of caspase-3(-like) proteases and apoptosis by cisplatin. In contrast, pretreatment of the cells with N-oleoylethanolamine (OE), a ceramidase inhibitor, potentiated apoptosis induced by cisplatin. Furthermore, OE enhanced sensitivity of the cisplatin-resistant cells to cisplatin. These results suggest that ceramide is closely implicated in apoptosis of glioma cells by cisplatin through activation of caspase-3(-like) proteases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 07:06:15