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Titolo:
Leaderless transcripts of the crenarchaeal hyperthermophile Pyrobaculum aerophilum
Autore:
Slupska, MM; King, AG; Fitz-Gibbon, S; Besemer, J; Borodovsky, M; Miller, JH;
Indirizzi:
Univ Calif Los Angeles, Dept Microbiol Mol Genet & Immunol, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA Univ Calif Los Angeles, Inst Mol Biol, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA Univ Calif Los Angeles, IGPP Ctr Astrobiol, Los Angeles, CA 90095 USA UnivCalif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA Georgia Inst Technol, Sch Biol, Atlanta, GA 30332 USA Georgia Inst Technol Atlanta GA USA 30332 Sch Biol, Atlanta, GA 30332 USA
Titolo Testata:
JOURNAL OF MOLECULAR BIOLOGY
fascicolo: 2, volume: 309, anno: 2001,
pagine: 347 - 360
SICI:
0022-2836(20010601)309:2<347:LTOTCH>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARCHAEON SULFOLOBUS-SOLFATARICUS; TRANSLATION INITIATION; MESSENGER-RNA; ESCHERICHIA-COLI; HALOBACTERIUM-HALOBIUM; MUTATIONAL ANALYSIS; DOWNSTREAM BOX; OPSIN GENE; TATA BOX; SEQUENCE;
Keywords:
Crenarchaea; leaderless transcripts; transcription; whole genome analysis; leaderless translation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Miller, JH Univ Calif Los Angeles, Dept Microbiol Mol Genet & Immunol, LosAngeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 , CA 90095 USA
Citazione:
M.M. Slupska et al., "Leaderless transcripts of the crenarchaeal hyperthermophile Pyrobaculum aerophilum", J MOL BIOL, 309(2), 2001, pp. 347-360

Abstract

We mapped transcription start sites for ten unrelated protein-encoding Pyrobaculum aerophilum genes by primer extension and S-1 nuclease mapping. Allof the mapped transcripts start at the computationally predicted translation start codons, two of which were supported by N-terminal protein sequencing. A whole genome computational analysis of the regions from -50 to +50 ntaround the predicted translation starts codons revealed a clear upstream pattern matching the consensus sequence of the archaeal TATA box located unusually close to the translation starts. For genes with the TATA boxes that best matched the con sensus sequence, the distance between the TATA box andthe translation start codon appears to be shorter than 30 nt. Two other promoter elements distinguished were also found unusually close to the translation start codons: a transcription initiator element with significant elevation of C and T frequencies at the -1 position and a BRE element with morefrequent A bases at position -29 to -32 (counting from the translation start site). We also show that one of the mapped genes is transcribed as the first gene of an operon. For a set of genes likely to be internal in operonsthe upstream signal extracted by computer analysis was a Shine-Dalgarno pattern matching the complementary sequence of P. aerophilum 16 S rRNA. Together these results suggest that the translation of proteins encoded by single genes or genes that are first in operons in the hyperthermophilic crenarchaeon P. aerophilum proceeds mostly, if not exclusively, through leaderlesstranscripts. Internal genes in operons are likely to undergo translation via a mechanism that is facilitated by ribosome binding to the Shine-Dalgarno sequence. (C) 2001 Academic Press.

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Documento generato il 29/11/20 alle ore 06:39:12