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Titolo:
Trace analysis of amikacin in commercial preparation by derivatization andHPLC
Autore:
Feng, CH; Lin, SJ; Wu, HL; Chen, SW;
Indirizzi:
Kaohsiung Med Univ, Sch Pharm, Kaohsiung 807, Taiwan Kaohsiung Med Univ Kaohsiung Taiwan 807 Sch Pharm, Kaohsiung 807, Taiwan
Titolo Testata:
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
fascicolo: 3, volume: 24, anno: 2001,
pagine: 381 - 392
SICI:
1082-6076(2001)24:3<381:TAOAIC>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; AMINOGLYCOSIDE ANTIBIOTICS; POSTCOLUMN DERIVATIZATION; BIOLOGICAL-FLUIDS; HUMAN PLASMA; ASSAYS; SERUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Chen, SW Kaohsiung Med Univ, Sch Pharm, Kaohsiung 807, Taiwan Kaohsiung Med Univ Kaohsiung Taiwan 807 , Kaohsiung 807, Taiwan
Citazione:
C.H. Feng et al., "Trace analysis of amikacin in commercial preparation by derivatization andHPLC", J LIQ CHR R, 24(3), 2001, pp. 381-392

Abstract

A simple and sensitive liquid chromatographic method has been developed for the determination of amikacin by derivatization. The method is based on the derivatization of amikacin with derivatizing agent, 1-naphthoyl chloride, in pyridine at 30 degreesC for 1 h. After derivatization reaction, a dimethylamine acetonitrile solution was added to the reaction mixture to eliminate the excess derivatizing agent. The derivative was analysed by HPLC on aDeita-Pak C-4 column with water-acetonitrile (15:85, v/v) as the mobile phase and detection at 295 nm. The parameters affecting the derivatization ofamikacin, including reaction temperature, reaction time, and the amount ofderivatizing agent, were investigated. The linear range of the method for the determination of amikacin was over 17-170 nmol/mL; the detection limit (signal to noise ratio = 5; injection volume, 20 muL) was 5 nmol/ml. Application of the method to the analysis of amikacin in commercial injections has proved satisfactory.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 11:51:09