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Titolo:
HPLC of acetyl-L-carnitine in human plasma by derivatization with p-bromophenacyl bromide
Autore:
Park, KM; Lee, MK; Park, YH; Woo, JS; Kim, CK;
Indirizzi:
Seoul Natl Univ, Coll Pharm, Natl Res Lab Bioact Delivery Syst, Seoul 151742, South Korea Seoul Natl Univ Seoul South Korea 151742 Syst, Seoul 151742, South Korea Hanmi Pharm Co Ltd, Cent Res Inst, Hwasung Gun 445910, Kyonggi Do, South Korea Hanmi Pharm Co Ltd Hwasung Gun Kyonggi Do South Korea 445910 South Korea
Titolo Testata:
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
fascicolo: 4, volume: 24, anno: 2001,
pagine: 555 - 563
SICI:
1082-6076(2001)24:4<555:HOAIHP>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; MASS-SPECTROMETRY; HUMAN-URINE; ESTER DERIVATIVES; ACYLCARNITINES; QUANTIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, CK Seoul Natl Univ, Coll Pharm, Natl Res Lab Bioact Delivery Syst, San 56-1, Seoul 151742, South Korea Seoul Natl Univ San 56-1 Seoul South Korea 151742 42, South Korea
Citazione:
K.M. Park et al., "HPLC of acetyl-L-carnitine in human plasma by derivatization with p-bromophenacyl bromide", J LIQ CHR R, 24(4), 2001, pp. 555-563

Abstract

A high performance liquid chromatographic method is developed for the determination of acetyl-L-carnitine (ALC), an endogenous carnitine derivative, in human plasma, e-Carnitine, which is the structural analogue of ALC, was used as an internal standard. The human plasma samples were treated with ninhydrin solution for the removal of amino acids and then extracted with methanol. After solid phase extraction on silica columns, samples were derivatized with p-bromophenacyl bromide in the presence of 18-crown-6. The derivative of ALC was quantified by high-performance liquid chromatography with UV detection at 260 nm. The retention time of ALC and e-carnitinewas about 27.5 and 22.3 min, respectively. The limit of quantitation was 0.22 nmol/mL, based on signal to noise ratio of 3. The accuracy deviation ofassay was less than 12.86%, and the intra-day and inter-day coefficients of variation (CV, %) were lower than 5.18 and 5.06%, respectively.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 01:16:06