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Titolo:
Solid-phase extraction with HPLC-DAD selective detection of amitriptyline and nortriptyline in human breast milk
Autore:
Caubet, MS; Millaret, A; Elbast, W; Brazier, JL;
Indirizzi:
Univ Montreal, Hop St Justine, Fac Pharm, Ctr Rech,Chaire Pharmaceut Famille Louis Boivin, Montreal, PQ H3C 3J7, Canada Univ Montreal Montreal PQ Canada H3C 3J7 in, Montreal, PQ H3C 3J7, Canada
Titolo Testata:
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
fascicolo: 8, volume: 24, anno: 2001,
pagine: 1181 - 1187
SICI:
1082-6076(2001)24:8<1181:SEWHSD>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAS-LIQUID-CHROMATOGRAPHY; HUMAN PLASMA; TRICYCLIC ANTIDEPRESSANTS; EXCRETION; SERUM; DRUGS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Caubet, MS Univ Montreal, Hop St Justine, Fac Pharm, Ctr Rech,Chaire Pharmaceut Famille Louis Boivin, CP 6128,Succursale Ctr Ville, Montreal, PQ H3C 3J7, Canada Univ Montreal CP 6128,Succursale Ctr Ville Montreal PQ Canada H3C 3J7
Citazione:
M.S. Caubet et al., "Solid-phase extraction with HPLC-DAD selective detection of amitriptyline and nortriptyline in human breast milk", J LIQ CHR R, 24(8), 2001, pp. 1181-1187

Abstract

A simple, rapid, highly selective and sensitive method was developed to determine concentrations of amitriptyline and nortriptyline in human breast milk, using high performance liquid chromatography (HPLC) with a diode-arraydetector (DAD). Drugs were effectively extracted from 250 muL of milk by asolid phase extraction procedure on a polymeric sorbent-C18-based cartridge. The separation was carried out on a Eclipse XDB-C18 reversed phase column (30 mm x 4.6 mm, 3.5 mum particle size) using the mobile phase composed of acetonitrile and ammonium acetate 0.1M (33/67, v/v). Imipramine was used as internal standard. Calibration functions were linear in the calibration range of 60-300 ng.mL(-1). The lower limit of detection was 1.9 ng.mL(-1) for both compounds. The recoveries were 92% for amitriptyline and 89% for nortriptyline. Since no method have been reported in the literature for the dosage of these two drugs in breast milk, this method could be useful, particularly, when evaluating the potential risk for the neonate during the lactation period.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 09:29:42