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Titolo:
Identification and determination of polyphenols in tea by liquid chromatography with multichannel electrochemical detection
Autore:
Long, H; Zhu, YX; Huang, YH; Coury, LA; Kissinger, PT;
Indirizzi:
Bioanalyt Syst Inc, W Lafayette, IN 47906 USA Bioanalyt Syst Inc W Lafayette IN USA 47906 nc, W Lafayette, IN 47906 USA
Titolo Testata:
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
fascicolo: 8, volume: 24, anno: 2001,
pagine: 1105 - 1114
SICI:
1082-6076(2001)24:8<1105:IADOPI>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
GREEN TEA; HUMAN PLASMA; RP-HPLC; CATECHINS; SEPARATION; ANTIOXIDANTS; COMPONENTS; EXTRACTION; INFUSIONS; FLAVANOLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Kissinger, PT Bioanalyt Syst Inc, 2701 Kent Ave, W Lafayette, IN 47906 USABioanalyt Syst Inc 2701 Kent Ave W Lafayette IN USA 47906 SA
Citazione:
H. Long et al., "Identification and determination of polyphenols in tea by liquid chromatography with multichannel electrochemical detection", J LIQ CHR R, 24(8), 2001, pp. 1105-1114

Abstract

A liquid chromatography method with multi-channel electrochemical detection is reported for the identification and determination of polyphenols in green tea, black tea, and extracted green tea powder. Following a simple aqueous extraction, (-)-epigallocatechin (EGC), (+)-catechin (C), (-)-epicatechin (EC), (-)-epigallocatechin gallate (EGCG), (-)-gallocatechin gallate (GCG), (-)-gallocatechin (GC), (-)-epicatechin gallate (ECG), and (-)catechin gallate (CG) were successfully separated by reversed phase liquid chromatography within 25 min. The detection limits (S/N=3) of the polyphenols studied were 0.1-1.5 ng/mLat a potential of +900mV. The calibration curve was linear over the range of 1-1000 ng/mL for EGG, C, EC, EGCG, GCG, and GC, 5-1000 ng/mL for ECG andCG. Using this method, the concentration of these eight polyphenols in five different brands of green tea, one brand of black tea, and extracted green tea powder were determined.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 21:26:31