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Titolo:
Immunohistochemical detection of human gastrointestinal glutathione peroxidase in normal tissues and cultured cells with novel mouse monoclonal antibodies
Autore:
Komatsu, H; Okayasu, I; Mitomi, H; Imai, H; Nakagawa, Y; Obata, F;
Indirizzi:
Kitasato Univ, Sch Allied Hlth Sci, Dept Immunol, Sagamihara, Kanagawa 2288555, Japan Kitasato Univ Sagamihara Kanagawa Japan 2288555 , Kanagawa 2288555, Japan Kitasato Univ, Sch Med, Dept Pathol, Sagamihara, Kanagawa 2288555, Japan Kitasato Univ Sagamihara Kanagawa Japan 2288555 , Kanagawa 2288555, Japan Kitasato Univ, Sch Pharmaceut Sci, Lab Hyg Chem, Minato Ku, Tokyo 108, Japan Kitasato Univ Tokyo Japan 108 Lab Hyg Chem, Minato Ku, Tokyo 108, Japan
Titolo Testata:
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
fascicolo: 6, volume: 49, anno: 2001,
pagine: 759 - 766
SICI:
0022-1554(200106)49:6<759:IDOHGG>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT TESTIS; EXPRESSION; SELENIUM; SEQUENCE; PROTEINS; IDENTIFICATION; ENZYME; TYPE-2; LINES; CDNA;
Keywords:
gastrointestinal glutathione peroxidase; monoclonal antibody; immunohistochemical staining; Western blotting; gastric carcinoma cell;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Komatsu, H Kitasato Univ, Sch Allied Hlth Sci, Dept Immunol, 1-15-1 Kitasato, Sagamihara, Kanagawa 2288555, Japan Kitasato Univ 1-15-1 Kitasato Sagamihara Kanagawa Japan 2288555
Citazione:
H. Komatsu et al., "Immunohistochemical detection of human gastrointestinal glutathione peroxidase in normal tissues and cultured cells with novel mouse monoclonal antibodies", J HIST CYTO, 49(6), 2001, pp. 759-766

Abstract

This is the first report to describe the successful detection of human gastrointestinal glutathione peroxidase in normal tissues by Western blotting and immunohistochemical staining techniques. Four hybridoma clones producing monoclonal antibodies (MAbs) against the human gastrointestinal glutathione peroxidase were established from mice immunized with a gastrointestinal glutathione peroxidase-derived peptide. The MAbs did not crossreact with other members of the glutathione peroxidase family, be it cellular glutathione peroxidase, phospholipid hydroperoxide glutathione peroxidase, or extracellular glutathione peroxidase. Although the MAbs were found to react with a24-kD protein in a Western blotting assay using gastric carcinoma cell extracts as antigen, they did not react with a B-lymphoblastoid cell extract. Immunohistochemical staining showed gastrointestinal glutathione peroxidaselocalized in the cytoplasm and in the nucleus of gastric carcinoma cells. Moreover, gastrointestinal glutathione peroxidase was detected in tissue extracts of human stomach, small intestine, large intestine, liver, and gallbladder by Western blotting, and its localization was immunohistochemically confirmed in the mucosal epithelia of the basal area of gastric pits and intestinal crypts.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:21:53