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Titolo:
Strain-dependent anterior segment neovascularization following intravitreal gene transfer of basic fibroblast growth factor (bFGF)
Autore:
Gupta, AR; Dejneka, NS; DAmato, RJ; Yang, ZX; Syed, N; Maguire, AM; Bennett, J;
Indirizzi:
Univ Penn, Dept Ophthalmol, Scheie Eye Inst, Inst Human Gene Therapy, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 e Therapy, Philadelphia, PA 19104 USA Harvard Univ, Sch Med, Childrens Hosp, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 ed, Childrens Hosp, Boston, MA 02115 USA Harvard Univ, Sch Med, Dept Ophthalmol, Boston, MA 02115 USA Harvard UnivBoston MA USA 02115 d, Dept Ophthalmol, Boston, MA 02115 USA
Titolo Testata:
JOURNAL OF GENE MEDICINE
fascicolo: 3, volume: 3, anno: 2001,
pagine: 252 - 259
SICI:
1099-498X(200105/06)3:3<252:SASNFI>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINANT ADENOASSOCIATED VIRUS; MEDIATED RETINAL TRANSDUCTION; IN-VIVO; DEGENERATION; PHOTORECEPTORS; ANGIOGENESIS; EXPRESSION; DELIVERY; LIGHT; RATS;
Keywords:
basic fibroblast growth factor; gene therapy; retinal degeneration; neovascularization; adenoviral vector; gene transfer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Bennett, J FM Kirby Ctr Mol Ophthalmol, Stellar Chance Labs 310, 422 CurieBlvd, Philadelphia, PA 19104 USA FM Kirby Ctr Mol Ophthalmol 422 Curie Blvd Philadelphia PA USA 19104
Citazione:
A.R. Gupta et al., "Strain-dependent anterior segment neovascularization following intravitreal gene transfer of basic fibroblast growth factor (bFGF)", J GENE MED, 3(3), 2001, pp. 252-259

Abstract

Background A promising strategy for delaying death of photoreceptor cells in retinal degenerative disease is to support survival of these cells through intraocular delivery of growth/neurotrophic factors. One factor that hasreceived great attention is basic fibroblast growth factor (bFGF; fgf-2), a known stimulator of angiogenesis. We evaluated the potential for neovascularization induced by adenovirus-mediated intravitreal delivery of bFGF. Methods Recombinant adenoviruses carrying the low molecular weight (18 kD)or the high molecular weight (22, 23 and 24 kD) forms of human bFGF, driven by the cytomegalovirus (CMV) promoter/enhancer, were prepared. Viruses were delivered to eyes of different strains of mice and rats through intravitreal injection. Contralateral eyes were injected with control virus carrying a reporter gene [green fluorescent protein (GFP) or lacZ]. Transgene expression was assessed by Western analysis and by immunohistochemistry. Neovascularization was evaluated in vivo and histologically at termination of theexperiment. Results Adenovirus-mediated delivery of the 18 kD form of bFGF resulted inanterior segment neovascularization in a strain-dependent fashion. Generation of new blood vessels was not observed after injection of the higher molecular weight forms of bFGF or of control solutions. Conclusion The low molecular weight form (18 kD) (but not the high molecular weight forms) of bFGF drives angiogenic response in the anterior segmentof specific strains of mice. Genetic modifiers may contribute to and/ or prevent neovascularization induced by bFGF. Copyright (C) 2001 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/01/20 alle ore 19:03:56