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Titolo:
Adenovirus-mediated gene transfer in canine eyes: a preclinical study for gene therapy of human uveal melanoma
Autore:
Andrawiss, M; Maron, A; Beltran, W; Opolon, P; Connault, E; Griscelli, F; Yeh, P; Perricaudet, M; Devauchelle, P;
Indirizzi:
Inst Gustave Roussy, UMR 1582, Aventis Gencell, F-94805 Villejuif, France Inst Gustave Roussy Villejuif France F-94805 , F-94805 Villejuif, France Ecole Natl Vet, Ophthalmol Dept, F-94704 Maisons Alfort, France Ecole NatlVet Maisons Alfort France F-94704 4704 Maisons Alfort, France
Titolo Testata:
JOURNAL OF GENE MEDICINE
fascicolo: 3, volume: 3, anno: 2001,
pagine: 228 - 239
SICI:
1099-498X(200105/06)3:3<228:AGTICE>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIAL GROWTH-FACTOR; IN-VIVO; REPORTER GENE; TRABECULAR MESHWORK; MALIGNANT-MELANOMA; OCULAR MELANOMAS; CANCER; ANGIOSTATIN; MODEL; CELLS;
Keywords:
uveal melanoma; dog eye; gene therapy; adenovirus; reporter gene; angiostatin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Andrawiss, M Univ Coll London, Windeyer Inst Med Sci, 46 Cleveland St, London W1P 6DB, England Univ Coll London 46 Cleveland St London England W1P 6DB land
Citazione:
M. Andrawiss et al., "Adenovirus-mediated gene transfer in canine eyes: a preclinical study for gene therapy of human uveal melanoma", J GENE MED, 3(3), 2001, pp. 228-239

Abstract

Background Melanomas of the uveal tract are the most common intraocular malignancies in adults, with an incidence of six cases per million adults peryear. Enucleation, which may enhance the dissemination of tumour cells into the systemic circulation is still required for eyes with large tumours. Gene therapy is proposed as a new therapeutic approach for uveal melanoma management. Methods The potential of adenovirus-mediated gene transfer to normal eyes of two laboratory Beagles and in an iris tumour of a Great Dome were evaluated. Replication-defective adenoviral vectors (Ad beta gal) were used to assess the feasibility, efficiency and safety of direct adenoviral delivery to the anterior chamber of normal eyes and to an iris tumour. The expressionof angiostatin into the aqueous humour following an adenoviral-mediated delivery of human angiostatin (AdK3) was also investigated. Results The ciliary body was the area preferentially transduced after adenoviral injection into the anterior chamber. It was also demonstrated that adirect intratumoral injection of a recombinant adenovirus efficiently transduces a canine uveal melanoma. Western blot analysis performed on the aqueous humour revealed that the expression of the angiostatin recombinant protein in the aqueous humour correlated with the dose of AdK3 administered. Lymphocyte infiltrates at the site of AdK3 injection indicated induction of astrong cellular immune response, and humoral immune responses developed inall three dogs. Conclusions The present study involving adenovirus-mediated gene transfer to dog eyes provides an essential basis for gene therapy treatment of uvealmelanoma-bearing patients. Copyright (C) 2001 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 03:17:26