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Titolo:
Development in electrofused conjugants of Tetrahymena thermophila
Autore:
Cole, ES; Virtue, MA; Stuart, KR;
Indirizzi:
St Olaf Coll, Dept Biol, Northfield, MN 55057 USA St Olaf Coll NorthfieldMN USA 55057 Dept Biol, Northfield, MN 55057 USA
Titolo Testata:
JOURNAL OF EUKARYOTIC MICROBIOLOGY
fascicolo: 3, volume: 48, anno: 2001,
pagine: 266 - 279
SICI:
1066-5234(200105/06)48:3<266:DIECOT>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAMMALIAN CELL FUSION; PARAMECIUM-TETRAURELIA; POSTZYGOTIC DEVELOPMENT; MEIOTIC CHROMOSOMES; MUTATIONAL ANALYSIS; NUCLEAR DEVELOPMENT; GENOMIC EXCLUSION; DNA; PYRIFORMIS; DIFFERENTIATION;
Keywords:
cell cycle; ciliates; conjugation; electrofusion; genomic exclusion; meiosis; mitosis; parabiosis; sexual reproduction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Cole, ES St Olaf Coll, Dept Biol, Northfield, MN 55057 USA St Olaf Coll Northfield MN USA 55057 l, Northfield, MN 55057 USA
Citazione:
E.S. Cole et al., "Development in electrofused conjugants of Tetrahymena thermophila", J EUKAR MIC, 48(3), 2001, pp. 266-279

Abstract

Electric shock can create parabiotic fusions of living Tetrahymena cells. In this study, cells were mated and successful pairs were electrofused witheither vegetatively growing cells or other mating pairs. In particular, weelectrofused pairs from normal [diploid X diploid] matings with vegetatively dividing cells in G- or M-phase of the cell cycle. We also fused [diploid x diploid] conjugants with mating pairs involving an aneuploid partner [diploid x "star"], which typically undergo an abortive conjugal pathway termed genomic exclusion. Using such parabiotic fusions we identified and characterized two developmentally critical landmarks: I) the "abort" signal, which is initiated in pairs with nuclear defects (this first becomes evident soon after the completion of Meiosis I or the beginning of Meiosis II): and 2) the "terminal commitment point", a developmental stage in normal [diploid x diploid] pairs after which conjugation no longer responds to a parabiotically transmitted abort signal (this correlates with the onset of the second postzygotic nuclear division). Finally we demonstrate that a conjugal-arrest-activity varies with the vegetative cell cycle, reaching its highest level of activity during M-phase and dropping just after cytokinesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 12:55:07