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Titolo:
Loss of sequences on the short arm of chromosome 17 is a late event in squamous carcinoma of the cervix
Autore:
Herrington, CS; Worsham, M; Southern, SA; Mackowiak, P; Wolman, SR;
Indirizzi:
Royal Liverpool Univ Hosp, Dept Pathol, Liverpool L69 3GA, Merseyside, England Royal Liverpool Univ Hosp Liverpool Merseyside England L69 3GA e, England Henry Ford Hosp, Dept Pathol, Detroit, MI 48202 USA Henry Ford Hosp Detroit MI USA 48202 , Dept Pathol, Detroit, MI 48202 USA Uniformed Serv Univ Hlth Sci, Dept Pathol, Bethesda, MD 20814 USA Uniformed Serv Univ Hlth Sci Bethesda MD USA 20814 Bethesda, MD 20814 USA
Titolo Testata:
JOURNAL OF CLINICAL PATHOLOGY-MOLECULAR PATHOLOGY
fascicolo: 3, volume: 54, anno: 2001,
pagine: 160 - 164
SICI:
1366-8714(200106)54:3<160:LOSOTS>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN PAPILLOMAVIRUS GENOTYPES; TUMOR-SUPPRESSOR GENE; UTERINE CERVIX; CELL CARCINOMA; HIGH-RISK; HETEROZYGOSITY; CYTOGENETICS; ONCOGENE; CANCER; P53;
Keywords:
cervix; fluorescence in situ hybridisation; chromosome 17; papillomavirus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Herrington, CS Royal Liverpool Univ Hosp, Dept Pathol, Liverpool L69 3GA, Merseyside, England Royal Liverpool Univ Hosp Liverpool Merseyside EnglandL69 3GA
Citazione:
C.S. Herrington et al., "Loss of sequences on the short arm of chromosome 17 is a late event in squamous carcinoma of the cervix", J CL PATH-M, 54(3), 2001, pp. 160-164

Abstract

Aims-To determine by fluorescence in situ hybridisation (FISH) whether deletion of D17S34, a subtelomeric probe for 17p, occurs in invasive squamous carcinoma of the cervix, and to determine the extent of such loss by analysis of the p53 and HER2/NEU genes. Methods-Fourteen invasive squamous cell carcinomas of the cervix were investigated by FISH for D17S34, p53, and HER2/NEU. Dual hybridisation of each probe with the chromosome 17 alpha satellite (D17Z1) probe was performed onparaffin wax embedded sections, and the fluorescence ratios of the paired signals were determined. Broad spectrum human papillomavirus (HPV) typing by ISH-I and GP5+/6+ polymerase chain reaction was also performed. Results-Twelve tumours were HPV positive, nine with HPV-16, and one each with types 18, 31, and 39. Loss of D17S34 was identified in four tumours, one of which was HPV negative. In one tumour, D17S34 loss was accompanied by loss of p53 only, suggesting that deletion was limited to the p arm. A second tumour showed simultaneous losses of all probes, indicative of whole chromosome 17 loss during tumour growth. The two remaining specimens showed loss of D17S34 only, diffuse in one, and localised within the tumour in the other. Aberrations of p53 or HER2/NEU were not seen independently of D17S33 loss, and loss did not correlate with HPV presence or type. Conclusions-These data show that D17S34 loss is prevalent, marking 28% of the invasive squamous carcinomas in this study. The observed intratumoral heterogeneity indicates that, at least in some cases, this loss occurs afterinvasion and is therefore a late event in the path of cervical carcinogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 12:17:13