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Titolo:
Use of vancomycin silica stationary phase in packed capillary electrochromatography II. Enantiomer separation of venlafaxine and O-desmethylvenlafaxine in human plasma
Autore:
Fanali, S; Rudaz, S; Veuthey, JL; Desiderio, C;
Indirizzi:
CNR, Ist Cromatog, Area Ric Roma, I-00016 Rome, Italy CNR Rome Italy I-00016 Ist Cromatog, Area Ric Roma, I-00016 Rome, Italy Univ Geneva, Lab Pharmaceut Analyt Chem, CH-1211 Geneva 4, Switzerland Univ Geneva Geneva Switzerland 4 lyt Chem, CH-1211 Geneva 4, Switzerland
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY A
fascicolo: 1, volume: 919, anno: 2001,
pagine: 195 - 203
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; IONIZATION MASS-SPECTROMETRY; CHIRAL SEPARATION; ENANTIOSELECTIVE DETERMINATION; BIOLOGICAL SAMPLES; REVERSED-PHASE; AMINO-ACIDS; ELECTROPHORESIS; CYCLODEXTRINS; SENSITIVITY;
Keywords:
stationary phases; CEC; enantiomer separation; electrochromatography; vancomycin; venlafaxine; O-desmethylvenlafaxine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Desiderio, C CNR, Ist Cromatog, Area Ric Roma, POB 10, I-00016 Rome, ItalyCNR POB 10 Rome Italy I-00016 a, POB 10, I-00016 Rome, Italy
Citazione:
S. Fanali et al., "Use of vancomycin silica stationary phase in packed capillary electrochromatography II. Enantiomer separation of venlafaxine and O-desmethylvenlafaxine in human plasma", J CHROMAT A, 919(1), 2001, pp. 195-203

Abstract

A capillary electrochromatography method, using vancomycin chiral stationary phase packed capillary, was optimized for the simultaneous chiral separation of the antidepressant drug venlafaxine and its main active metabolite O-desmethylvenlafaxine. Simultaneous baseline enantiomeric separation of the two compounds was obtained using a mobile phase composed of 100 mM ammonium acetate buffer pH 6/water/acetonitrile (5:5:90, v/v). The electrokineticinjection for sample introduction provided a limit of quantitation for both the compounds of 0.05 mug/ml racemate concentration suitable for the analysis of venlafaxine and metabolite in biological samples. The acetonitrile mobile phase concentration was found to modulate the analytes elution times, the enantiomeric resolution and the efficiency of the separation. The column was tested for repeatability and linearity showing RSD values (%) in the range of 0.13-0.24, 2.47-3.66 and 1.35-2.50 for migration time, sample/internal standard peak area ratio and enantiomeric resolution, respectively and correlation coefficients higher than 0.9990. The method was applied to the analysis of clinical samples of patients under depression therapy showing a stereoselective metabolism for venlafaxine. (C) 2001 Elsevier Science B. V. All rights reserved.

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Documento generato il 09/04/20 alle ore 07:41:52