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Titolo:
Expression of leukocyte adhesion molecules by endothelial cells seeded on various polymer surfaces
Autore:
Imbert, E; Poot, AA; Figdor, CG; Feijen, J;
Indirizzi:
Univ Twente, Sect Polymer Chem & Biomat, Inst Biomed Technol, NL-7500 AE Enschede, Netherlands Univ Twente Enschede Netherlands NL-7500 AE 500 AE Enschede, Netherlands Univ Twente, Fac Chem Technol, NL-7500 AE Enschede, Netherlands Univ Twente Enschede Netherlands NL-7500 AE 500 AE Enschede, Netherlands
Titolo Testata:
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
fascicolo: 3, volume: 56, anno: 2001,
pagine: 376 - 381
SICI:
0021-9304(20010905)56:3<376:EOLAMB>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN EXPRESSION; POLYETHYLENE TEREPHTHALATE; TRANSENDOTHELIAL MIGRATION; IN-VITRO; CONTACT; DACRON; NEUTROPHIL; BINDING; GRAFTS; TRANSPLANTATION;
Keywords:
endothelial cells; leukocyte adhesion molecules; polymer surfaces; flow cytometry; immunofluorescence microscopy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Feijen, J Univ Twente, Sect Polymer Chem & Biomat, Inst Biomed Technol, POB 217, NL-7500 AE Enschede, Netherlands Univ Twente POB 217 Enschede Netherlands NL-7500 AE Netherlands
Citazione:
E. Imbert et al., "Expression of leukocyte adhesion molecules by endothelial cells seeded on various polymer surfaces", J BIOMED MR, 56(3), 2001, pp. 376-381

Abstract

Although endothelial cell seeding in small-diameter vascular prostheses significantly improves graft survival, the detachment of adherent endothelialcells after the restoration of circulation remains one of the major obstacles. Because in vivo experiments indicate that leukocyte infiltration is involved in endothelial cell loss, we hypothesize that seeded endothelial cells become activated and express leukocyte adhesion molecules and cytokines because of an interaction with the underlying polymer surface. The aim of this study was to investigate the expression of the leukocyte adhesion molecules ICAM-1, VCAM-1, PECAM-1, and E-selectin by cultured human umbilical vein endothelial cells (HUVECs) and human adipose microvascular endothelial cells (HAMVECs). The cells were seeded on tissue culture poly(styrene) and the vascular graft materials Dacron and Teflon. The results of this study indicate that the expression of leukocyte adhesion molecules by cultured endothelial cells is mainly affected by the endothelial cell origin, that is, umbilical vein or adipose tissue. Expressions of both ICAM-1 and E-selectin by HUVECs and HAMVECs are characterized by the presence of two cell populations with distinct levels of expression. With respect to endothelial cell seeding in vascular prostheses, the increased expression of E-selectin by microvascular endothelial cells deserves further attention. (C) 2001 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/21 alle ore 18:26:36