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Titolo:
Binding of adeno-associated virus type 5 to 2,3-linked sialic acid is required for gene transfer
Autore:
Walters, RW; Yi, SMP; Keshavjee, S; Brown, KE; Welsh, MJ; Chiorini, JA; Zabner, J;
Indirizzi:
Univ Iowa, Coll Med, Dept Internal Med, Iowa City, IA 52242 USA Univ IowaIowa City IA USA 52242 pt Internal Med, Iowa City, IA 52242 USA Univ Iowa, Coll Med, Dept Physiol & Biophys, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 ysiol & Biophys, Iowa City, IA 52242 USA Univ Iowa, Coll Med, Dept Otolaryngol, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 ept Otolaryngol, Iowa City, IA 52242 USA Univ Iowa, Coll Med, Howard Hughes Med Inst, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 Hughes Med Inst, Iowa City, IA 52242 USA Univ Toronto, Toronto Lung Transplant Program, Toronto, ON M5G 2C4, CanadaUniv Toronto Toronto ON Canada M5G 2C4 ogram, Toronto, ON M5G 2C4, Canada NIDCR, Gene Therapeut Branch, NIH, Bethesda, MD 20892 USA NIDCR Bethesda MD USA 20892 Therapeut Branch, NIH, Bethesda, MD 20892 USA NHLBI, Hematol Branch, NIH, Bethesda, MD 20892 USA NHLBI Bethesda MD USA 20892 , Hematol Branch, NIH, Bethesda, MD 20892 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 23, volume: 276, anno: 2001,
pagine: 20610 - 20616
SICI:
0021-9258(20010608)276:23<20610:BOAVT5>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINANT ADENOASSOCIATED VIRUS; CILIATED AIRWAY EPITHELIA; INFLUENZA-C VIRUS; TRACHEAL EPITHELIUM; CANINE PARVOVIRUS; AAV VECTOR; FACTOR-IX; CELLS; RECEPTOR; TRANSDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Zabner, J Univ Iowa, Coll Med, Dept Internal Med, 500 EMRB, Iowa City, IA 52242 USA Univ Iowa 500 EMRB Iowa City IA USA 52242 owa City, IA 52242 USA
Citazione:
R.W. Walters et al., "Binding of adeno-associated virus type 5 to 2,3-linked sialic acid is required for gene transfer", J BIOL CHEM, 276(23), 2001, pp. 20610-20616

Abstract

Recombinant adeno-associated viruses (AAV) are promising gene therapy vectors. Whereas AAV serotype 2-mediated gene transfer to muscle has partially replaced factor IX deficiency in hemophilia patients, its ability to mediate gene transfer to the lungs for cystic fibrosis is hindered by lack of apical receptors. However, AAV serotype 5 infects human airway epithelia from the lumenal surface. We found that in contrast to AAV2, the apical membraneof airway epithelia contains abundant high affinity receptors for AAV5. Binding and gene transfer with AAV5 was abolished by genetic or enzymatic removal of sialic acid from the cell surface. Furthermore, binding and gene transfer to airway epithelia was competed by lectins that specifically bind 2,3-linked sialic acid. These observations suggest that 2,3-linked sialic acid is either a receptor for AAV5 or it is a necessary component of a receptor complex. Further elucidation of the receptor for this virus should enhance understanding of parvovirus biology and expand the therapeutic targets for AAV vectors.

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Documento generato il 05/07/20 alle ore 15:34:16