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Titolo:
Independent repression of a GC-rich housekeeping gene by Sp1 and MAZ involves the same cis-elements
Autore:
Song, J; Ugai, H; Kanazawa, I; Sun, K; Yokoyama, KK;
Indirizzi:
RIKEN, Inst Phys & Chem Res, Tsukuba Inst, Tsukuba, Ibaraki 3050074, JapanRIKEN Tsukuba Ibaraki Japan 3050074 Inst, Tsukuba, Ibaraki 3050074, Japan Univ Tokyo, Fac Med, Inst Brain Res, Dept Neurol, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 in Res, Dept Neurol, Tokyo 1130033, Japan China Med Univ, Dept Med Genet, Shenyang 110001, Peoples R China China MedUniv Shenyang Peoples R China 110001 g 110001, Peoples R China
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 23, volume: 276, anno: 2001,
pagine: 19897 - 19904
SICI:
0021-9258(20010608)276:23<19897:IROAGH>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
ZINC-FINGER PROTEIN; TRANSCRIPTION FACTOR SP1; C-MYC GENE; HISTONE DEACETYLASE; MOLECULAR-CLONING; CDNA CLONE; PROMOTER; BINDING; DNA; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Yokoyama, KK RIKEN, Inst Phys & Chem Res, Tsukuba Inst, 3-1-1 Koyadai, Tsukuba, Ibaraki3050074, Japan RIKEN 3-1-1 Koyadai Tsukuba Ibaraki Japan 3050074 0074, Japan
Citazione:
J. Song et al., "Independent repression of a GC-rich housekeeping gene by Sp1 and MAZ involves the same cis-elements", J BIOL CHEM, 276(23), 2001, pp. 19897-19904

Abstract

The transcription factors Spl and MAZ ((M) under bar yc-(a) under bar ssociated (z) under bar inc finger protein) contain several zinc finger motifs,and each functions as both a positive and a negative regulator of gene expression. In this study, we characterized the extremely GC-rich promoter of the human gene for MAZ, which is known as a housekeeping gene. Unique symmetrical motifs in the promoter region (nucleotides -383 to -334) were essential for the expression of the gene for MAZ, whereas an upstream silencer element (nucleotides -784 to -612) was found to act in a position-dependent but orientation-independent manner. Spl and MAZ bound to the same cis-elements in the GC-rich promoter, apparently sharing DNA-binding sites. The relative extent of binding of Spl and MAZ to these cis-elements corresponded to the extent of negative regulation of the expression of the gene for MAZ in various lines of cells. Furthermore, novel repressive do mains in both Spl (amino acids 622-788) and MAZ (amino acids 127-292) were identified. Suppression by Spl and suppression by MAZ were independent phenomena; histone deacetylases were involved in the autorepression by MAZ itself, whereas DNA methyltransferase 1 was associated with suppression by Spl. Our results indicate that both deacetylation and methylation might be involved in the regulation of expression of a single gene via the actions of different zinc finger proteins that bind to the same cis-elements.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 10:18:29