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Titolo:
Thiol-oxidizing agent diamide and acidic pH enhance lipid peroxidation of rat heart mitochondria and cardiolipin-cytochrome c complex
Autore:
Iwase, H; Sakurada, K; Ikegaya, H; Hatanaka, K; Takeichi, H; Kobayashi, M; Yoshida, K;
Indirizzi:
Univ Tokyo, Dept Forens Med, Grad Sch Med, Bunkyo Ku, Tokyo 1130033, JapanUniv Tokyo Tokyo Japan 1130033 Sch Med, Bunkyo Ku, Tokyo 1130033, Japan
Titolo Testata:
IUBMB LIFE
fascicolo: 1, volume: 51, anno: 2001,
pagine: 39 - 43
SICI:
1521-6543(200101)51:1<39:TADAAP>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINOLEIC-ACID; LIPOXYGENASE ACTIVITY; BOVINE HEART; INJURY; CALCIUM; PHOSPHOLIPIDS; HEMOPROTEINS; REPERFUSION; DYSFUNCTION; LEUKOTOXIN;
Keywords:
cardiolipin; cytochrome c; free radical; linoleic acid; lipid peroxidation; mitochondria; thiol;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Iwase, H Univ Tokyo, Dept Forens Med, Grad Sch Med, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1130033, Japan Univ Tokyo 7-3-1 Hongo Tokyo Japan 1130033 Tokyo 1130033, Japan
Citazione:
H. Iwase et al., "Thiol-oxidizing agent diamide and acidic pH enhance lipid peroxidation of rat heart mitochondria and cardiolipin-cytochrome c complex", IUBMB LIFE, 51(1), 2001, pp. 39-43

Abstract

In this study, we investigated lipid peroxidation in rat heart mitochondria hydrolyzed by phospholipase A2 (PLA2) and lipid peroxidation in a mitochondrial-mimetic lipid peroxidation system, where phospholipids such as cardiolipin (CL) and cytochrome c (Cyt c) were first mixed together and then PLA2 and calcium chloride were added to the mixture (CL Cyt c-PLA2 system). Production of hydroperoxy and hydroxy compounds of linoleic acid (LA)(1) in the mixture was measured by high performance liquid chromatography, The ratio of the total amount of hydroperoxy and hydroxy compounds of LA to that ofLA was calculated as an index for lipid peroxidation (1000 x mol/mol). Theindex for lipid peroxidation in the rat heart mitochondria hydrolyzed by PLA2 at the physiological pH of 7.4 was 22.8 +/- 2.2 (mean +/- SD, n = 4) and that at the acidic pH of 6.7 was 41.8 +/- 2,0, In the presence of the thiol (SH)-oxidizing agent diamide, the index was 47.0 +/- 2.6 (pH 7,4), In the CG-Cyt c-PLA2 system, lipid peroxidation seemed to be due to three mechanisms: (1) oxidation of the LA (nonreleased form) constituent of CL by Cyt c(oxidation of CL by Cyt c); (2) oxidation of free LA, released from CL, involving the oxidation of CL by Cyt c (free LA oxidation by the CL-Cyt c complex); and (3) oxidation of free LA, released from CL, by Cyt c and calciumions (LA-Cyt c-Ca system). The lipid peroxidation of the CL-Cyt c-PLA2 system was also enhanced by the addition of diamide and by an acidic pH of 6,7, The fact that the SH-oxidizing agent enhanced the lipid peroxidation in the CG Cyt c-PLA2 system suggested that SH groups in the hemoprotein played an inhibitory role in lipid peroxidation in the system.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 10:00:57