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Titolo:
Contribution of the residue Glu9, Glu46, and Phe49 to the biological activity of insulin-like growth factor-1
Autore:
Chen, H; Feng, YM;
Indirizzi:
Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China Chinese Acad Sci Shanghai Peoples R China 200031 200031, Peoples R China
Titolo Testata:
IUBMB LIFE
fascicolo: 1, volume: 51, anno: 2001,
pagine: 33 - 37
SICI:
1521-6543(200101)51:1<33:COTRGG>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR-I; RECEPTOR-BINDING; RELAXIN; HYBRID; PEPTIDE; CHAIN; CDNA; EVOLUTION; AMPHIOXUS; PRECURSOR;
Keywords:
IGF-1; insulin; insulin structural motif; scaffold molecule;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Feng, YM Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China Chinese Acad Sci 320 Yue Yang Rd Shanghai Peoples R China 200031
Citazione:
H. Chen e Y.M. Feng, "Contribution of the residue Glu9, Glu46, and Phe49 to the biological activity of insulin-like growth factor-1", IUBMB LIFE, 51(1), 2001, pp. 33-37

Abstract

Three recombinant single-chain insulin mutants, [B10Glu]Ins/ IGF-1(C), [A5Glu]PIP, and [A8Tyr]PIP were prepared by means of protein engineering, In the three mutants, the residue at position 10 of insulin B chain and positions 5 and 8 of insulin A chain were substituted by the corresponding residueGlu9, Glu46, and an aromatic residue at 49 of IGF-1, respectively. The growth-promoting activity of [B18Glu]Ins/IGF-1(C) is 58-fold higher than insulin and of [A5Glu]PIP, and [A8Tyr]PIP is 2.5- and 4.7-fold higher than PIP, respectively, measured using a mouse mammary tumor cell line GR2H6 in vitro, The results show that the residues Glu9, Glu46, and Phe49, especially theGlu9, play an important role in the growth-promoting function of IGF-1, Based on the results, we suggest that the insulin could be used as a scaffoldto investigate the contributions of residues of IGF-1 to its biological function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 03:27:03