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Titolo:
Anti-alpha 2 integrin antibody induces secretion and activation of 72-kDa progelatinase by human fibroblasts
Autore:
Ishibashi, Y; Ito, H; Seyama, Y; Kubota, S;
Indirizzi:
Univ Tokyo, Dept Physiol Chem & Metab, Grad Sch Med, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 Sch Med, Bunkyo Ku, Tokyo 1130033, Japan
Titolo Testata:
IUBMB LIFE
fascicolo: 1, volume: 51, anno: 2001,
pagine: 25 - 31
SICI:
1521-6543(200101)51:1<25:A2IAIS>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
MATRIX METALLOPROTEASE-2; SIGNAL TRANSDUCTION; FIBROSARCOMA CELLS; TISSUE INHIBITOR; IV COLLAGENASE; EXPRESSION; INVASION; MMP-2; CONCOMITANT; STROMELYSIN;
Keywords:
72-kDa gelatinase; extracellular matrix proteins; p42 MAPK; anti-alpha 2 integrin antibody; human fibroblasts;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Kubota, S Univ Tokyo, Dept Physiol Chem & Metab, Grad Sch Med, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1130033, Japan Univ Tokyo 7-3-1 Hongo Tokyo Japan 1130033 Tokyo 1130033, Japan
Citazione:
Y. Ishibashi et al., "Anti-alpha 2 integrin antibody induces secretion and activation of 72-kDa progelatinase by human fibroblasts", IUBMB LIFE, 51(1), 2001, pp. 25-31

Abstract

We examined the effects of the extracellular matrix proteins fibronectin, laminin, and matrigel on 72-kDa progelatinase secretion by human lip fibroblasts (KD) and skin fibroblasts (MRC9 and HSF). By gelatin zymography, 1.7 /- 0.2-fold, 1.7 +/- 0.2-fold, and 1.8 +/- 0.3-fold increases were observed in 72-kDa progelatinase secretion from KD cells treated with fibronectin,laminin, and matrigel, respectively, Laminin and matrigel, but not fibronectin, stimulated 72-kDa progelatinase secretion from HSF cells, Fibronectin, laminin, and matrigel did not stimulate 72-kDa progelatinase secretion byMRC9 cells. Anti-alpha2 integrin antibody-stimulated 72-kDa progelatinase secretion and induced the 62-kDa activated form of 72-kDa progelatinase by KD cells. Activated p42 MAP kinase (MAPK) expression was down-regulated by anti-alpha2 integrin antibody, Anti-alpha2 integrin antibody stimulated 72-kDa progelatinase secretion by HSF cells without inducing the 62-kDa activated form, The data suggest that interaction between fibroblasts and extracellular matrix components via alpha2 integrin plays an important role in regulating secretion and activation of 72-kDa gelatinase and that down-regulation of activated p42 MAPK may be involved in the 72-kDa progelatinase activation mechanism.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 22:12:46