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Titolo:
Frog oocytes: A living test tube for studies on metabolic regulation
Autore:
Ureta, T; Preller, A; Kessi, E;
Indirizzi:
Univ Chile, Fac Ciencias, Dept Biol, Santiago, Chile Univ Chile SantiagoChile ile, Fac Ciencias, Dept Biol, Santiago, Chile Univ Chile, Dept Ciencias Biol Anim, Fac Ciencias Vet & Pecuarias, Santiago, Chile Univ Chile Santiago Chile Fac Ciencias Vet & Pecuarias, Santiago, Chile
Titolo Testata:
IUBMB LIFE
fascicolo: 1, volume: 51, anno: 2001,
pagine: 5 - 10
SICI:
1521-6543(200101)51:1<5:FOALTT>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEPATIC GLYCOGEN-SYNTHESIS; AMPHIBIAN OOCYTES; INDIRECT PATHWAY; GLUCOSE; AVAILABILITY;
Keywords:
frog oocytes; flux control coefficients; glucose metabolism; glycogen synthesis; pentose-P pathway;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Ureta, T Univ Chile, Fac Ciencias, Dept Biol, Santiago, Chile Univ Chile Santiago Chile Ciencias, Dept Biol, Santiago, Chile
Citazione:
T. Ureta et al., "Frog oocytes: A living test tube for studies on metabolic regulation", IUBMB LIFE, 51(1), 2001, pp. 5-10

Abstract

This review is intended to illustrate how live frog oocytes may be advantageously used to address the study of some problems of in vivo glucose metabolism. Glucose microinjected into the cells is preferentially committed to glycogen synthesis. We present evidence showing that both the direct and indirect pathways for polysaccharide deposition are operative in oocytes, A small amount of the injected glucose (<5%) is released as labeled CO2 mainlythrough the pentose-P pathway. Coinjection of NADP(+) and glucose significantly stimulates (CO2)-C-14 production, half-maximal stimulation being obtained at 0.13 mM, Finally, we show the use of frog oocytes to measure in vivo the control coefficient of hexokinase on glycogen synthesis and the pentose-P pathway. A value of 0.7 was found for the control coefficient of hexokinase on glycogen synthesis, while the enzyme has no control at all over the pentose-P pathway. Therefore, the frog oocyte may be used as a living test tube for the study of almost any metabolic process of interest.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:38:48