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Titolo:
Release of mediators by DMSO-differentiated HL-60 cells exposed to electric interferential current and the requirement of biochemical prestimulation
Autore:
Sontag, W;
Indirizzi:
Forschungszentrum Karlsruhe, Inst Med Tech & Biophys, D-76021 Karlsruhe, Germany Forschungszentrum Karlsruhe Karlsruhe Germany D-76021 Karlsruhe, Germany
Titolo Testata:
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
fascicolo: 6, volume: 77, anno: 2001,
pagine: 723 - 734
SICI:
0955-3002(200106)77:6<723:ROMBDH>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
STOCHASTIC RESONANCE; ELECTROMAGNETIC-FIELDS; PEPTIDE RECEPTOR; PHORBOL ESTER; MESSENGER-RNA; CYCLIC-AMP; G-PROTEINS; INTERLEUKIN-8; KINASE; PHOSPHORYLATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Sontag, W Forschungszentrum Karlsruhe, Inst Med Tech & Biophys, Postfach 3640, D-76021 Karlsruhe, Germany Forschungszentrum Karlsruhe Postfach 3640 Karlsruhe Germany D-76021
Citazione:
W. Sontag, "Release of mediators by DMSO-differentiated HL-60 cells exposed to electric interferential current and the requirement of biochemical prestimulation", INT J RAD B, 77(6), 2001, pp. 723-734

Abstract

Purpose : Controversial results have been obtained with respect to the influence of biochemical prestimulation upon the action of electromagnetic fields on mammalian cells. Examined in detail has been the influence of a modulated low-frequency (interferential) current upon the release of four mediators by DMSO-differentiated HL-60 cells with and without prestimulation by the chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine (fMLP). Materials and methods : Human promyelocytes (HL-60) cells differentiated to granulocytes by dimethylsulphoxide were exposed to a so-called interferential current (IFC) in a suspension culture. The influence of IFC treatment,an amplitude-modulated 4000 Hz current used in therapeutic applications, on the release of prostaglandine E-2 (PGE(2)), leukotriene B-4 (LTB4), thromboxane B-2 (TXB2) and interleukin 8 (IL-8) was investigated. Effects of a static magnetic field were also studied. Results: In no case were significant differences between exposed and sham-exposed samples observed after 15 min exposure to selected modulation frequencies (0-125 Hz, 250 muA cm(-2)). Therefore, the influence of prestimulation with different concentrations of fMLP on the release of the four mediators was investigated. fMLP alone enhanced the release of PGE(2), LTB4 TXB2 and IL-8 with half of the maximum (ED50) effective concentration of similar to 10 nm. Prestimulation with various concentrations of fMLP and subsequentexposure to IFC 125 Hz (15 min, 250 muA cm(-2)) had no effect at low or high concentrations of fMLP, while in the middle range of concentration of similar to 100 pm fMLP a significant enhancement of PGE(2), LTB4 and IL-8 wasobserved. This synergistic effect of fMLP and IFC was examined in detail in prestimulated cells (100 pm fMLP) by varying the exposure time, current density and modulation frequency in the range 0-50 Hz. Window effects for the release of PGE(2), LTB4 and IL-8 were obtained for exposure time, currentdensity and modulation frequency whereas, in the release of TXB2, no fieldeffect was detected. Conclusion : The study has demonstrated the synergistic action of chemicalstimulation and electric current exposure, and the existence of windows for cellular activation status, exposure time, current density and modulationfrequency.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 11:22:42