Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Early checking of genetic stability of cork oak somatic embryos by AFLP analysis
Autore:
Hornero, J; Martinez, I; Celestino, C; Gallego, FJ; Torres, V; Toribio, M;
Indirizzi:
IMIA, Madrid 28800, Spain IMIA Madrid Spain 28800IMIA, Madrid 28800, Spain INIA, Dept Mejora Genet & Biotecnol, Unidad Marcadores Mol, Madrid, Spain INIA Madrid Spain net & Biotecnol, Unidad Marcadores Mol, Madrid, Spain
Titolo Testata:
INTERNATIONAL JOURNAL OF PLANT SCIENCES
fascicolo: 4, volume: 162, anno: 2001,
pagine: 827 - 833
SICI:
1058-5893(200107)162:4<827:ECOGSO>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
QUERCUS-SUBER; SOMACLONAL VARIATION; CELL-CULTURES; EMBRYOGENESIS; FIDELITY; PLANTS; RAPD; L.;
Keywords:
AFLP; cork oak; DNA markers; in vitro culture; Quercus suber; somaclonal variation; somatic embryogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Toribio, M IMIA, Finca El Encin,Apartado 127, Madrid 28800, Spain IMIA Finca El Encin,Apartado 127 Madrid Spain 28800 800, Spain
Citazione:
J. Hornero et al., "Early checking of genetic stability of cork oak somatic embryos by AFLP analysis", INT J PL SC, 162(4), 2001, pp. 827-833

Abstract

Early checking of the genetic stability of tissue culture-derived plants is necessary to obtain all the potential benefits of clonal forestry. Previous work in Quercus suber L. using random amplified polymorphic DNA (RAPD) markers showed no genetic variation among somatic embryos within embryogeniclines of zygotic origin. Genetic fingerprinting based on amplified fragment length polymorphisms (AFLP) allows the direct analysis of variation at the entire DNA level by generating more reproducible markers than RAPDs. To confirm the absence of genetic variation within six embryogenic lines of zygotic origin, six primer pairs were selected out of 48 combinations of primers for revealing up to 512 AFLP markers, 301 of them (58.8%) being polymorphic. The mean number of markers per genotype was 375. Again differences were recorded among embryogenic lines, even between those that arose from half-sib zygotic embryos, but no variation was observed among somatic embryos within embryogenic lines. To check variation in embryogenic lines raised from nonembryonic tissues, somatic embryogenesis was induced in expanding leaves collected from sprouts originating in three mature cork oak. DNA was extracted from leaves and from somatic embryos derived from each tree, and three primer pairs showed 165, 110, and 108 markers per genotype/tree, respectively. In one tree, AFLP patterns generated from leaves and somatic embryoswere identical, but variation was detected in samples from the other two trees. Although the level of genetic variation detected in these lines (5.6%and 7.3% of polymorphism, respectively) is lower than that recorded for half sibs of cork oak (25%-31%), its influence on phenotypic variation needs further assessment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/10/20 alle ore 09:57:47