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Titolo:
Clonal chromosomal aberrations in simian virus 40-transfected human thyroid cells and in derived tumors developed after in vitro irradiation
Autore:
Zitzelsberger, H; Bruch, J; Smida, J; Hieber, L; Peddie, CM; Bryant, PE; Riches, AC; Fung, JL; Weier, HUG; Bauchinger, M;
Indirizzi:
Lawrence Berkeley Lab, Div Life Sci, Berkeley, CA USA Lawrence Berkeley Lab Berkeley CA USA ab, Div Life Sci, Berkeley, CA USA Univ Calif San Francisco, Dept Obstet Gynecol, Reprod Sci Grp, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ St Andrews, Sch Biol, St Andrews, Fife, Scotland Univ St Andrews St Andrews Fife Scotland iol, St Andrews, Fife, Scotland Univ Munich, Inst Radiat Biol, Munich, Germany Univ Munich Munich Germany iv Munich, Inst Radiat Biol, Munich, Germany GSF Forschungszentrum Umwelt & Gesundheit GMBH, Inst Radiobiol, Neuherberg, Germany GSF Forschungszentrum Umwelt & Gesundheit GMBH Neuherberg Germany rmany
Titolo Testata:
INTERNATIONAL JOURNAL OF CANCER
fascicolo: 3, volume: 96, anno: 2001,
pagine: 166 - 177
SICI:
0020-7136(20010620)96:3<166:CCAISV>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPARATIVE GENOMIC HYBRIDIZATION; RADIATION-INDUCED TRANSFORMATION; COPY NUMBER CHANGES; EPITHELIAL-CELLS; CELLULAR SENESCENCE; NEOPLASTIC TRANSFORMATION; INDEFINITE DIVISION; IONIZING-RADIATION; ALPHA-PARTICLES; SOLID TUMORS;
Keywords:
in vitro model cell system; radiation-induced tumorigenesis; chromosomal aberration; comparative genomic hybridization; spectral karyotyping;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Zitzelsberger, H GSF Forschungszentrum Umwelt & Gesundheit GMBH, Inst Strahlenbiol, D-85758Oberschleissheim, Germany GSF Forschungszentrum Umwelt & Gesundheit GMBH Oberschleissheim Germany D-85758
Citazione:
H. Zitzelsberger et al., "Clonal chromosomal aberrations in simian virus 40-transfected human thyroid cells and in derived tumors developed after in vitro irradiation", INT J CANC, 96(3), 2001, pp. 166-177

Abstract

In vitro model cell systems are important tools for studying mechanisms ofradiation-induced neoplastic transformation of human epithelial cells. In our study, the human thyroid epithelial cell line HTori-3 was analyzed cytogenetically following exposure to different doses of alpha- and gamma -irradiation and subsequent tumor formation in at athymic nude mice. Combining results from G-banding, comparative genomic hybridization, and spectral karyotyping, chromosome abnormalities could be depicted in the parental line HTori-3 and in nine different HTori lines established from the developed tumors. A number of chromosomal aberrations were found to be characteristic forsimian virus 40 immortalization and/or radiation-induced transformation ofhuman thyroid epithelial cells. Common chromosomal changes in cell lines originating from different irradiation experiments were loss of 8q23 and 13cen-q21 as well as gain of 1q32-qter and 2q11.2-q14.1. By comparison of chromosomal aberrations in cell lines exhibiting a different tumorigenic behavior, cytogenetic markers important for the tumorigenic process were studied. It appeared that deletions on chromosomes 9q32-q34 and 7q21-q31 as cs ell as an increased copy number of chromosome 20 were important for the tumorigenic phenotype. A comparative breakpoint analysis of the marker chromosomesfound and those observed in radiation-induced childhood thyroid tumors from Belarus revealed a coincidence for a number of chromosome bands. Thus, the data support the usefulness of the established cell system as an in vitromodel to study important steps during radiation-induced malignant transformation in human thyroid cells. (C) 2001 Wiley-Liss, Inc.

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Documento generato il 01/10/20 alle ore 00:49:56