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Titolo:
Effects of suplatast tosilate (IPD Capsules((R))) on the production of active oxygen by neutrophils and of IL-8 by mononuclear cells
Autore:
Tohda, Y; Kubo, H; Haraguchi, R; Iwanaga, T; Fukuoka, M;
Indirizzi:
Kinki Univ, Sch Med 377 2, Dept Internal Med 4, Osaka 5890014, Japan KinkiUniv Osaka Japan 5890014 Dept Internal Med 4, Osaka 5890014, Japan
Titolo Testata:
INTERNATIONAL IMMUNOPHARMACOLOGY
fascicolo: 6, volume: 1, anno: 2001,
pagine: 1183 - 1187
SICI:
1567-5769(200106)1:6<1183:EOST(C>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; EPITHELIAL-CELLS; GENE-EXPRESSION; ASTHMA; INTERLEUKIN-8; CYTOKINES; INFLAMMATION; IPD-1151T; RELEASE; IGE;
Keywords:
bronchial asthma; neutrophils; mononuclear cells; active oxygen species; IL-8; suplatast tosilate;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Tohda, Y Kinki Univ, Sch Med 377 2, Dept Internal Med 4, Osaka 5890014, Japan Kinki Univ Osaka Japan 5890014 rnal Med 4, Osaka 5890014, Japan
Citazione:
Y. Tohda et al., "Effects of suplatast tosilate (IPD Capsules((R))) on the production of active oxygen by neutrophils and of IL-8 by mononuclear cells", INT IMMUNO, 1(6), 2001, pp. 1183-1187

Abstract

In bronchial asthma, eosinophils and neutrophils are activated, so that the production of active oxygen species increases, causing airway epithelial injury. Suplatast tosilate (IPD Capsules (R)) is a novel immunomodulating antiallergic drug that acts against bronchial asthma through a new mechanism. To evaluate the effects of suplatast tosilate on mononuclear cell-mediatedIL-8 production, and neutrophil-mediated active oxygen species production at sites of inflammation, we collected peripheral blood from healthy subjects and separated the neutrophils as well as mononuclear cells. Suplatast tosilate was added at a concentration of 1 X 10(-6), 1 X 10(-7) or 1 X 10(-8)M, and cells were incubated for 10 min at 37 degreesC. Then, the neutrophils were stimulated with fMLP, and luminol-dependent chemiluminescence (LDCL) was measured, while IL-8 production was determined with an ELISA kit. Suplatast tosilate (1 X 10(-6) M) inhibited neutrophil-mediated active oxygen species production by 12.4% in terms of the peak, and by 16% in terms of the integral value. Moreover, ii. significantly inhibited mononuclear cell-mediated IL-8 production at concentrations of 1 X 10(-6), 1 X 10(-7) and 1 X 10(-8) M, in a concentration-dependent manner. This study indicated that suplatast tosilate may inhibit neutrophil infiltration by suppressing monocyte-mediated IL-8 production, and it may also inhibit the activation of neutrophils at sites of inflammation. These results suggest the possibility that suplatast tosilate may not only be of benefit for asthma, but may alsoprevent or control pulmonary fibrosis or emphysema, for which no effectivetreatment is presently available. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 10:29:14