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Titolo:
Induction of CD4(+) regulatory T cells by 12-O-tetradecanoylphorbol 13-acetate in mice Part 1: The strain difference in this induction
Autore:
Negoro, T; Iinuma, F; Tobe, T; Watanabe, M;
Indirizzi:
Showa Univ, Sch Pharmaceut Sci, Dept Med Informat, Shinagawa Ku, Tokyo 1428555, Japan Showa Univ Tokyo Japan 1428555 ormat, Shinagawa Ku, Tokyo 1428555, Japan Teikyo Univ, Fac Pharmaceut Sci, Dept Analyt Chem, Kanagawa 1990195, JapanTeikyo Univ Kanagawa Japan 1990195 Analyt Chem, Kanagawa 1990195, Japan
Titolo Testata:
INTERNATIONAL IMMUNOPHARMACOLOGY
fascicolo: 6, volume: 1, anno: 2001,
pagine: 1153 - 1163
SICI:
1567-5769(200106)1:6<1153:IOCRTC>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
DELAYED-TYPE HYPERSENSITIVITY; 12-TETRADECANOYLPHORBOL 13-ACETATE; CONTACT SENSITIVITY; FOOTPAD REACTION; PHORBOL ESTER; TH1 CLONES; TNF-ALPHA; SUPPRESSION; INTERLEUKIN-4; RESPONSES;
Keywords:
DTH; immunomodulation; TPA; DIF;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Negoro, T Showa Univ, Sch Pharmaceut Sci, Dept Med Informat, Shinagawa Ku,1-5-8 Hatanodai, Tokyo 1428555, Japan Showa Univ 1-5-8 Hatanodai Tokyo Japan 1428555 o 1428555, Japan
Citazione:
T. Negoro et al., "Induction of CD4(+) regulatory T cells by 12-O-tetradecanoylphorbol 13-acetate in mice Part 1: The strain difference in this induction", INT IMMUNO, 1(6), 2001, pp. 1153-1163

Abstract

12-O-Tetradecanoylphorbol 13-acetate (TPA), an activator of signal transduction, induced antigen-nonspecific regulatory T (Tr) cells for delayed-typehypersensitivity (DTH) in the spleen. A marked strain difference in the induction of Tr cells was observed when the study was performed by using the several strains of mice at 6-8 weeks old. TPA painting induced CD4(+)Tr cells in C3H/He (H-2(k)), C3H/HeN (H-2(k)), DBA/2 (H-2(d)) and AKR/N (H-2(k)) mice, but not in C57BL/6 (H-2(b)), C57BL/10 (H-2(b)), BALB/c (H-2(d)) and A/J (H-2(a)). Regulatory cells were also induced by incubating spleen cells from unprimed mice with TPA in vitro and were seemed to act by the production of soluble factor(s). A downregulatory activity of the soluble factor(s) was abrogated by SXC-1 (anti-IL-10 monoclonal antibody), but not by SXC-2 (anti-IL-10 monoclonal antibody) and anti-IL-4. For purification of the factor(s), we established the T cell hybridoma 4C5-1 by the fusion of spleen cells from TPA-treated C3H/He mice with AKR thymoma Bw5147 cells. The 4C5-1 cells secrete the factor(s) which can inhibit DTH response. The inhibitory activity of the factor(s) could be neutralized by SXC-1, but not by SXC-2, anti-IL-4, anti-IL-6 and anti-TGF-beta antibodies. The factor(s) could not affect the proliferation and IFN-gamma production of alpha s1-casein-specific 3D20 Th1 cells. The factor(s) termed DIF (DTH Inhibitory Factor) may be a novel cytokine, since they have reduced the footpad swelling response by local injection, and have no immune crossreactivity with the DTH regulatory cytokines and no inhibitory activity for in vitro Th1 response. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 01/10/20 alle ore 16:21:48