Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Aqueous extract isolated from Platycodon grandiflorum elicits the release of nitric oxide and tumor necrosis factor-alpha from murine macrophages
Autore:
Choi, CY; Kim, JY; Kim, YS; Chung, YC; Seo, JK; Jeong, HG;
Indirizzi:
Chosun Univ, Dept Pharm, Gong Ku, Kwangju 501759, South Korea Chosun UnivKwangju South Korea 501759 g Ku, Kwangju 501759, South Korea Chosun Univ, Res Ctr Proteineous Mat, Gong Ku, Kwangju 501759, South KoreaChosun Univ Kwangju South Korea 501759 g Ku, Kwangju 501759, South Korea Korea Res Inst Chem Technol, Taejon 305606, South Korea Korea Res Inst Chem Technol Taejon South Korea 305606 05606, South Korea Coll Chinju, Dept Food Nutr, Chinju, South Korea Coll Chinju Chinju South Korea nju, Dept Food Nutr, Chinju, South Korea Jangsaeng Doraji Co Ltd, Dept R&D, Chinju, South Korea Jangsaeng Doraji CoLtd Chinju South Korea ept R&D, Chinju, South Korea
Titolo Testata:
INTERNATIONAL IMMUNOPHARMACOLOGY
fascicolo: 6, volume: 1, anno: 2001,
pagine: 1141 - 1151
SICI:
1567-5769(200106)1:6<1141:AEIFPG>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE MACROPHAGES; SYNTHASE GENE; CELLS; LIPOPOLYSACCHARIDE; POLYSACCHARIDE; CYTOTOXICITY; MECHANISMS; EXPRESSION; MEDIATORS; INDUCTION;
Keywords:
Platycodon grandiflorum; macrophages; nitric oxide; tumor necrosis factor-alpha;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Jeong, HG Chosun Univ, Dept Pharm, Gong Ku, 375 Seosuk Dong, Kwangju 501759, South Korea Chosun Univ 375 Seosuk Dong Kwangju South Korea 501759 th Korea
Citazione:
C.Y. Choi et al., "Aqueous extract isolated from Platycodon grandiflorum elicits the release of nitric oxide and tumor necrosis factor-alpha from murine macrophages", INT IMMUNO, 1(6), 2001, pp. 1141-1151

Abstract

Herbal medicines are increasingly being utilized to treat a wide variety of disease processes. Aqueous extract from the root of Platycodon grandiflorum A. DC (Campanulaceae),, Changkil (CK), is reported to have antitumor andimmunomodulatory activities; however, the mechanism underlying its therapeutic effect is not known. In the present study we examined the effects of CK on the release of nitric oxide (NO) and tumor necrosis factor-alpha (TNF-alpha), and on the gene expression of iNOS and TNF-alpha in mouse macrophages. CK elicited a dose-dependent increase in NO and TNF-alpha production incultured macrophages. CK significantly affected secretion at concentrations of more than 5 mug/ml, and its maximum effect was at concentration of 100mug/ml. Reverse transcription polymerase chain reaction showed that increases in NO and TNF-alpha secretion were due to an increase in inducible NO synthase mRNA and TNF-alpha mRNA, respectively. Transient expression assays with NF-kappaB binding sites linked to the luciferase gene revealed that CK-induced increase of inducible NO synthase mRNA and TNF-alpha mRNA were mediated by the NF-kappaB transcription factor complex. These results demonstrate that CK stimulates NO and TNF-alpha release and is able to upregulate iNOS and TNF-alpha expression through NF-kappaB transactivation and this maybe a mechanism whereby this herbal medicine elicits its therapeutic effects. (C) 2001 Elsevier Science B.V, All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 16:19:03