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Titolo:
Preparation and functional properties of polyclonal and monoclonal antibodies to murine MD-1
Autore:
Hadidi, S; Yu, K; Chen, ZQ; Gorczynski, RM;
Indirizzi:
Toronto Gen Hosp, Transplant Res Div, Toronto, ON M5G 2C4, Canada Toronto Gen Hosp Toronto ON Canada M5G 2C4 v, Toronto, ON M5G 2C4, Canada Univ Toronto, Dept Surg, Toronto, ON M5S 1A8, Canada Univ Toronto TorontoON Canada M5S 1A8 Surg, Toronto, ON M5S 1A8, Canada Univ Toronto, Dept Immunol, Toronto, ON M5S 1A8, Canada Univ Toronto Toronto ON Canada M5S 1A8 munol, Toronto, ON M5S 1A8, Canada
Titolo Testata:
IMMUNOLOGY LETTERS
fascicolo: 2, volume: 77, anno: 2001,
pagine: 97 - 103
SICI:
0165-2478(20010601)77:2<97:PAFPOP>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
PORTAL VENOUS IMMUNIZATION; EXPRESSION; ACTIVATION; IMMUNITY; OX-2; PROLONGATION; MOLECULE; HOMOLOG; CELLS; GENE;
Keywords:
MD-1; anti-MD-1 antibodies; cytokine production;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Gorczynski, RM Toronto Gen Hosp, Transplant Res Div, CCRW 2-855,200 Elizabeth St, Toronto, ON M5G 2C4, Canada Toronto Gen Hosp CCRW 2-855,200 Elizabeth St Toronto ON Canada M5G 2C4
Citazione:
S. Hadidi et al., "Preparation and functional properties of polyclonal and monoclonal antibodies to murine MD-1", IMMUNOL LET, 77(2), 2001, pp. 97-103

Abstract

Rabbits, rats and hamsters were immunized with KLH-coupled synthetic peptide sequences of the murine MD-1 molecule. Serum from immunized animals bound in Western gels to a 25 KDa protein extracted from LPS stimulated mouse spleen cells, as did a rat hybridoma (SH1.2.47) prepared from peptide-immunized rats. CHO cells transfected with a plasmid cDNA construct encoding murine MD-1, the target antigen for the antibodies in question, were also stained (in FACS) by the same antibodies. Patching and capping of the antigen(s)detected by any one of these sera abolished binding of all antibodies in subsequent FAGS analysis, consistent with the hypothesis that they all detected the same antigen. In a final study to assess the possible involvement of MD-1 in regulation of cell activation for cytokine production following allostimulation, we found that all of the antibodies inhibited IL-2. and IFNgamma production, while enhancing IL-4 and IL-10 production, in mixed leukocyte reactions (MLR) in vitro. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 03:07:12