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Titolo:
Calcium buffering of resting, voltage-dependent Ca2+ influx by sarcoplasmic reticulum in femoral arteries from spontaneously hypertensive rats at prehypertensive stage
Autore:
Asano, M; Nomura, Y;
Indirizzi:
Nagoya City Univ, Sch Med, Dept Pharmacol, Mizuho Ku, Nagoya, Aichi 4678601, Japan Nagoya City Univ Nagoya Aichi Japan 4678601 Nagoya, Aichi 4678601, Japan
Titolo Testata:
Hypertension research
fascicolo: 3, volume: 24, anno: 2001,
pagine: 271 - 282
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASCULAR SMOOTH-MUSCLE; POTENT VASOCONSTRICTOR ACTIONS; CYCLOPIAZONIC ACID; K+ CHANNELS; MESENTERIC-ARTERY; MYOGENIC TONE; THAPSIGARGIN; RYANODINE; STATE; CELLS;
Keywords:
sarcoplasmic reticulum; superficial buffer barrier function; cytosolic Ca2+ level; Ca2+ influx via L-type Ca2+ channels; prehypertensive SHR artery;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Asano, M Nagoya City Univ, Sch Med, Dept Pharmacol, Mizuho Ku, Mizuhocho, Nagoya, Aichi 4678601, Japan Nagoya City Univ Mizuhocho Nagoya Aichi Japan 4678601 8601, Japan
Citazione:
M. Asano e Y. Nomura, "Calcium buffering of resting, voltage-dependent Ca2+ influx by sarcoplasmic reticulum in femoral arteries from spontaneously hypertensive rats at prehypertensive stage", HYPERTENS R, 24(3), 2001, pp. 271-282

Abstract

We examined the Ca2+-buffering function of the sarcoplasmic reticulum (SR)in the resting state of arteries from spontaneously hypertensive rats (SHR) at a prehypertensive stage. Differences in the effects of cyclopiazonic acid (CPA) and thapsigargin, agents that inhibit SR Ca2+-ATPase, and of ryanodine, which depletes SR Ca2+, on tension and cellular Ca2+ level were assessed in endothelium-denuded strips of femoral arteries from 4-week-old SHR and normotensive Wistar-Kyoto rats (WKY), Addition of CPA, thapsigargin or ryanodine to the resting state of the strips caused an elevation of cytosolic Ca2+ level and a contraction in both WKY and SHR. These responses were larger in SHR than in WKY, The contractions were inhibited strongly by 100 nM nifedipine or 3 muM verapamil and were abolished by Ca2+-free solution. Nifedipine, verapamil or Ca2+-free solution itself caused a relaxation from the resting state of SHR strips, but not from that of WKY strips. The resting Ca2+ influx in arteries measured by a 5-min incubation with Ca-45 was significantly larger in SHR than in WKY. This influx was decreased by 10 muM CPA or 10 muM ryanodine in both WKY and SHR, These results suggest that in the resting state of the femoral artery from 4-week-old SHR, the greater part of the increased Ca2+ influx via L-type Ca2+ channels is buffered by Ca2 uptake into the SR, while some Ca2+ reaches the myofilaments, resulting in the maintenance of resting tone.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/10/20 alle ore 16:53:46