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Titolo:
Differential effects among thiazolidinediones on the transcription of thromboxane receptor and angiotensin II type 1 receptor genes
Autore:
Sugawara, A; Takeuchi, K; Uruno, A; Ikeda, Y; Arima, S; Sato, K; Kudo, M; Taniyama, Y; Ito, S;
Indirizzi:
Tohoku Univ, Grad Sch Med, Dept Med, Div Nephrol Endocrinol & Vasc Med, Sendai, Miyagi 9808574, Japan Tohoku Univ Sendai Miyagi Japan 9808574 ed, Sendai, Miyagi 9808574, Japan
Titolo Testata:
Hypertension research
fascicolo: 3, volume: 24, anno: 2001,
pagine: 229 - 233
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASCULAR SMOOTH-MUSCLE; LOW-DENSITY-LIPOPROTEIN; TROGLITAZONE; OXIDATION; GAMMA; EXPRESSION; LIGAND; AGENTS; RATS;
Keywords:
troglitazone; pioglitazone; rosiglitazone; PPAR-gamma; alpha-tocopherol; antioxidant;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Sugawara, A Tohoku Univ, Grad Sch Med, Dept Med, Div Nephrol Endocrinol & Vasc Med, Sendai, Miyagi 9808574, Japan Tohoku Univ Sendai Miyagi Japan 9808574 Miyagi 9808574, Japan
Citazione:
A. Sugawara et al., "Differential effects among thiazolidinediones on the transcription of thromboxane receptor and angiotensin II type 1 receptor genes", HYPERTENS R, 24(3), 2001, pp. 229-233

Abstract

Peroxisome proliferator-activated receptor (PPAR)-gamma ligands thiazolidinediones (TZDs) have recently been reported to be anti-hypertensive and anti-atherosclerotic. We have previously shown that one of the TZDs troglitazone significantly suppressed the transcription of both thromboxane receptor (TXR) and angiotensin II type 1 receptor(AT1R) genes in vascular smooth muscle cells (VSMCs) by activating PPAR-gamma, In the present study, we compared the effects of troglitazone and other TZDs on the transcription of thesegenes. TXR and AT1R mRNAs in rat VSMCs were determined by semi-quantitative RT-PCR, Luciferase chimeric constructs containing either the 989-bp rat TXR gene promoter or the 1,969-bp rat AT1R gene promoter were transiently transfected into VSMCs, The cells were incubated with troglitazone, RS-1455 Ia derivative of troglitazone which does not contain the hindered phenol resembling alpha -tocopherol), pioglitazone, or rosiglitazone for 12 h before harvesting. mRNA expression levels of TXR and AT1R were significantly decreased by troglitazone in contrast to rosiglitazone, TXR gene and AT1R gene transcription was significantly suppressed by troglitazone in a dose-dependent manner, while RS-1455 was less potent. Pioglitazone and rosiglitazone weakly suppressed the transcription of both genes in a manner almost similar to F18-1455. We have shown that troglitazone suppresses transcription of both the TXR and AT1R genes more potently than other TZDs. The structure of troglitazone and RS-1455 is identical except the hindered phenol, which is recently recognized to function as an antioxidant, Moreover, we have shown that the potency for activating PPAR-gamma is almost identical between troglitazone and RS-1455, We therefore speculate that the strong transcriptionalsuppression of the TXR and AT1R genes by troglitazone may be mediated in part by its antioxidant effect.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 06:16:05