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Titolo:
Facilitated glucose transporters play a crucial role throughout mouse preimplantation embryo development
Autore:
Leppens-Luisier, G; Urner, F; Sakkas, D;
Indirizzi:
Univ Hosp Geneva, Dept Obstet & Gynaecol, Clin Steril, CH-1211 Geneva 4, Switzerland Univ Hosp Geneva Geneva Switzerland 4 ril, CH-1211 Geneva 4, Switzerland
Titolo Testata:
HUMAN REPRODUCTION
fascicolo: 6, volume: 16, anno: 2001,
pagine: 1229 - 1236
SICI:
0268-1161(200106)16:6<1229:FGTPAC>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
METABOLIC CONTROL; HEXOSE-TRANSPORT; CULTURE-MEDIUM; IN-VITRO; EXPRESSION; PYRUVATE; INVITRO; OOCYTES; FERTILIZATION; BLASTOCYSTS;
Keywords:
glucose analogues; glucose transporters; preimplantation mouse embryos;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Sakkas, D Yale Univ, Sch Med, Dept Obstet & Gynecol, 333 Cedar St,POB 208063, New Haven, CT 06520 USA Yale Univ 333 Cedar St,POB 208063 New Haven CT USA 06520 520 USA
Citazione:
G. Leppens-Luisier et al., "Facilitated glucose transporters play a crucial role throughout mouse preimplantation embryo development", HUM REPR, 16(6), 2001, pp. 1229-1236

Abstract

The role of glucose fluctuates during preimplantation mouse embryo development, indicating that a specific interplay exists between glucose metabolism and uptake. In this study, attempts were made to characterize the role ofthe Na+-coupled active and the facilitated glucose transporters (GLUT) during preimplantation development by using specific glucose analogues and transport inhibitors and by examining the expression of GLUT1. One-cell outbred mouse embryos were cultured in medium M16 (5.5 mmol/l glucose), M16 without glucose (M16-G), M16-G + 2-deoxyglucose, M16-G + 3-O-methylglucose, M16 phlorizin and M16 + phloretin and development to the blastocyst stage assessed. The absence of glucose, or the presence of 3-O-methylglucose, which is taken up but not metabolized, did not inhibit blastocyst development. 2-Deoxyglucose, which is phosphorylated but not metabolized, inhibited blastocyst development. Culture in M16 supplemented with phlorizin, an inhibitor of Na+-coupled active glucose transport did not inhibit blastocyst formation. Phloretin had no effect on the cleavage of two-cell embryos to the four-cell stage, but inhibited the morula/blastocyst transition, Both phloretin and phlorizin inhibited glucose uptake in two-cell embryos. Finally, GLUT1 expression was Ill-fold less in blastocysts cultured in M16 compared to in-vivo blastocysts and those cultured in M16-G, The results show that both types of glucose transporters influence preimplantation embryo development and that the embryo has an innate ability to control the uptake of glucose byregulating the expression of GLUT1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 21:01:52