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Titolo:
Immunolocalization of NuMA and phosphorylated proteins during the cell cycle in human breast and prostate cancer cells as analyzed by immunofluorescence and postembedding immunoelectron microscopy
Autore:
Gobert, GN; Hueser, CN; Curran, EM; Sun, QY; Glinsky, VV; Welshons, WV; Eisenstark, A; Schatten, H;
Indirizzi:
Univ Missouri, Dept Vet Pathobiol, Columbia, MO 65211 USA Univ Missouri Columbia MO USA 65211 Vet Pathobiol, Columbia, MO 65211 USA Canc Res Ctr, Columbia, MO 65211 USA Canc Res Ctr Columbia MO USA 65211Canc Res Ctr, Columbia, MO 65211 USA Univ Missouri, Dept Vet Biomed Sci, Columbia, MO 65211 USA Univ Missouri Columbia MO USA 65211 et Biomed Sci, Columbia, MO 65211 USA Univ Missouri, Dept Biochem, Columbia, MO 65211 USA Univ Missouri Columbia MO USA 65211 Dept Biochem, Columbia, MO 65211 USA
Titolo Testata:
HISTOCHEMISTRY AND CELL BIOLOGY
fascicolo: 5, volume: 115, anno: 2001,
pagine: 381 - 395
SICI:
0948-6143(200105)115:5<381:IONAPP>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
MITOTIC APPARATUS PROTEIN; NUCLEAR MATRIX PROTEINS; SPINDLE; CENTROSOME; APOPTOSIS; LOCALIZATION; MITOSIS; NUCLEOSKELETON; ORGANIZATION; INSTABILITY;
Keywords:
breast cancer; nuclear proteins; cytoskeleton; cell cycle; centrosomes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Schatten, H Univ Missouri, Dept Vet Pathobiol, 1600 E Rollins St, Columbia, MO 65211 USA Univ Missouri 1600 E Rollins St Columbia MO USA 65211 5211 USA
Citazione:
G.N. Gobert et al., "Immunolocalization of NuMA and phosphorylated proteins during the cell cycle in human breast and prostate cancer cells as analyzed by immunofluorescence and postembedding immunoelectron microscopy", HISTOCHEM C, 115(5), 2001, pp. 381-395

Abstract

The formation of mitotic centrosomes is a complex process in which a number of cellular proteins translocate to mitotic poles and play a critical role in the organization of the mitotic apparatus. The 238-kDa nuclear mitoticapparatus protein NuMA is one of the important proteins that plays a significant role in this process. NuMA resides in the nucleus during interphase and becomes transiently associated with mitotic centrosomes after multiple steps of phosphorylations. The role of NuMA in the interphase nucleus is not well known but it is clear that NuMA responds to external signals (such as hormones) that induce cell division, or heat shock that induces apoptosis. In order to determine the function of NuMA it is important to study its localization. Here we report on nuclear organization of NuMA during the cellcycle in estrogen responsive MCF-7 breast cancer cells and in androgen responsive LNCaP prostate cancer cells using immunoelectron microscopy, and oncorrelation to MPM-2 monoclonal phosphoprotein antibody. These results show that NuMA is present in speckled and punctate form associated with distinct material corresponding to a speckled or punctate immunofluorescence appearance in the nucleus while MPM-2 is uniformly dispersed in the nucleus. Atprophase NuMA disperses in the cytoplasm and associates with microtubules while MPM-2 is uniformly distributed in the cytoplasm. During metaphase or anaphase anti-NuMA labeling is associated with spindle fibers. During telophase NuMA relocates to electron-dense areas around chromatin and finally tothe reconstituted nuclei. These results demonstrate NuMA organization in MCF-7 and LNCaP cells in the log phase of cell culture growth.

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Documento generato il 22/09/20 alle ore 10:16:29