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Titolo:
Immunohistochemical expression of molecular markers in an avian model: A potential model for preclinical evaluation of agents for ovarian cancer chemoprevention
Autore:
Rodriguez-Burford, C; Barnes, MN; Berry, W; Partridge, EE; Grizzle, WE;
Indirizzi:
Univ Alabama, Dept Obstet & Gynecol, Birmingham, AL 35233 USA Univ Alabama Birmingham AL USA 35233 & Gynecol, Birmingham, AL 35233 USA Univ Alabama, Dept Pathol, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 ept Pathol, Birmingham, AL 35294 USA Auburn Univ, Dept Poultry Sci, Auburn, AL 36849 USA Auburn Univ Auburn ALUSA 36849 v, Dept Poultry Sci, Auburn, AL 36849 USA
Titolo Testata:
GYNECOLOGIC ONCOLOGY
fascicolo: 3, volume: 81, anno: 2001,
pagine: 373 - 379
SICI:
0090-8258(200106)81:3<373:IEOMMI>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMORS; RATS; ADENOCARCINOMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Rodriguez-Burford, C Univ Alabama, Dept Obstet & Gynecol, Birmingham, AL 35233 USA Univ Alabama Birmingham AL USA 35233 m, AL 35233 USA
Citazione:
C. Rodriguez-Burford et al., "Immunohistochemical expression of molecular markers in an avian model: A potential model for preclinical evaluation of agents for ovarian cancer chemoprevention", GYNECOL ONC, 81(3), 2001, pp. 373-379

Abstract

Objective. A significant obstacle confronting the evaluation of potential chemopreventive compounds in ovarian carcinoma is the absence of an animal model of spontaneous ovarian carcinogenesis. A potential model of adenocarcinoma has been described in the laying hen (Gallus domesticus). The purposeof this study was to evaluate the immunohistochemical expression of available antibodies that have been utilized in chemoprevention studies in this potential model of epithelial carcinoma. Methods. Two hundred 2-year-old hens were sacrificed at Auburn University in accordance with IUACUC guidelines. Of these hens, 8 animals were thoughtgrossly to have ovarian carcinoma and ascites. The tumors from these 8 hens were fixed in neutral-buffered formalin and processed to paraffin blocks. Hematoxylin and eosin stains were used to document the histologic presenceof adenocarcinoma. Immunohistochemical evaluation for expression of antigen was performed using the following antibodies: CA125, CEA, cytokeratin, EGFR, erbB-2, Ki-67, Lewis Y, p27, PCNA, Tag 72, TGF-alpha, Muc 1, and Muc 2. Results. Upon microscopic examination by a pathologist eight specimens were documented as adenocarcinomas. Several antibodies to antigens that are frequently expressed in human ovarian cancer were cross-reactive in the laying hen. Of these, cytokeratin AE1/AE3, pan cytokeratin, EGFR, Lewis Y, CEA, Tag 72, and erbB-2 stained the chicken carcinomas. EGFR and p185(erbB-2) stained diffusely, and cytokeratin AE1/AE3, pan cytokeratin, Lewis Y, CEA, and Tag 72 were focally positive in the tumor. The aforementioned antibodies which have been useful as surrogate endpoints in chemoprevention trials andwhich also stained the chicken carcinomas included PCNA, p27, and TGF-alpha Antibodies that were not cross-reactive include CA 125, Ki-67, Muc 1, andMuc 2. Conclusion. The data presented in this pilot study support the potential utility of an avian model of spontaneously arising adenocarcinoma in which to study chemopreventive agents. More importantly, the influence of chemoprevention protocols on the expression of relevant antigens can be determined using available antibodies that are cross-reactive in this model. Thus, changes in the phenotypic expression of surrogate endpoint biomarkers as identified by cross-reactive antibodies can aid in the development of chemoprevention trials for human ovarian cancer. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 02:11:38