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Titolo:
A new radioimmunoassay to measure rat insulin-like growth factor binding protein-4 (IGFBP-4) in serum, wound fluid and conditioned media
Autore:
Chelius, D; Spencer, EM;
Indirizzi:
Calif Pacific Med Res Inst, Lab Growth & Dev, San Francisco, CA 94114 USA Calif Pacific Med Res Inst San Francisco CA USA 94114 cisco, CA 94114 USA
Titolo Testata:
GROWTH HORMONE & IGF RESEARCH
fascicolo: 1, volume: 11, anno: 2001,
pagine: 49 - 57
SICI:
1096-6374(200102)11:1<49:ANRTMR>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
OSTEOBLAST-LIKE CELLS; FACTOR-I; FACTOR BINDING-PROTEIN-4; FIBROBLAST GROWTH; MOLECULAR-CLONING; IDENTIFICATION; PROTEOLYSIS; MECHANISM; CULTURES;
Keywords:
IGFBP-4; radioimmunoassay; rodent; serum; age dependence;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Spencer, EM Calif Pacific Med Res Inst, Lab Growth & Dev, Castro & Duboce St, San Francisco, CA 94114 USA Calif Pacific Med Res Inst Castro & Duboce St San Francisco CA USA 94114
Citazione:
D. Chelius e E.M. Spencer, "A new radioimmunoassay to measure rat insulin-like growth factor binding protein-4 (IGFBP-4) in serum, wound fluid and conditioned media", GROWTH H I, 11(1), 2001, pp. 49-57

Abstract

Insulin-like growth factor binding protein-4 (IGFBP-4) is, like the other five IGFBPs, a critical regulator of the activity of insulin-like growth factor-I (IGF-I) and IGF-II. Because of the possible role of IGFBP-4 in multiple systems including reproduction, pregnancy, bone formation and wound healing, we developed a radioimmunoassay (RIA) to measure the concentration ofIGFBP-4, in the serum, extracellular fluid and conditioned media of cells of rodents so that these animal models could be better exploited. Rat IGFBP-4 was expressed in Escherichia coli and purified to homogeneity. The recombinant material was used to raise a rabbit polyclonal antibody against rat IGFBP-4 and for iodination and standards. The RIA developed was sensitive to less than 1 ng/mL of rat IGFBP-4 and IGF-I did not interfere. There was no cross-reactivity with other rat IGFBPs on immuno-Western analysis of serum and wound fluid. IGFBP-4 from mouse serum did cross-react in our assay; however, serum from horse, pig or human showed no crossreaction. Human IGFBP-1 and IGFBP-3 showed a very weak cross-reactivity. Serum IGFBP-4 levels showed no gender difference but did reveal a significant 66% increase in older rats. During the course of wound healing, which is IGF-I dependent, IGFBP-4 showed no changes. In conclusion an RIA for rat IGFBP-4 has been developed that specifically measures the concentration of IGFBP-4 in the sera, extracellular fluid and conditioned media of rodents. With this assay, the role of IGFBP-4 and IGFs in growth, development and the function of multiple systems can be further investigated using rodent models. (C) 2001 Harcourt Publishers Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 08:18:51